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| | {{STRUCTURE_2hwt| PDB=2hwt | SCENE= }} | | {{STRUCTURE_2hwt| PDB=2hwt | SCENE= }} |
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| - | '''NMR solution structure of the Master-Rep protein nuclease domain (2-95) from the Faba Bean Necrotic Yellows Virus'''
| + | ===NMR solution structure of the Master-Rep protein nuclease domain (2-95) from the Faba Bean Necrotic Yellows Virus=== |
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| - | ==Overview==
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| - | Nanoviruses are a family of plant viruses that possess a genome of multiple circular single-stranded DNA (ssDNA) components and are strikingly similar in their replication mode to the plant geminiviruses and to the circoviruses that infect birds or mammals. These viruses multiply by rolling circle replication using virus-encoded multifunctional replication initiator proteins (Rep proteins) that catalyze the initiation of replication on a double-stranded DNA (dsDNA) intermediate and the resolution of the ssDNA into circles. Here we report the solution NMR three-dimensional structure of the endonuclease domain from the master Rep (M-Rep) protein of faba bean necrotic yellows virus (FBNYV), a representative of the nanoviruses. The domain comprises amino acids 2-95 (M-Rep2-95), and its global fold is similar to those previously described for the gemini- and circovirus Rep endonuclease domains, consisting of a central 5-stranded antiparallel beta-sheet covered on one side by an alpha-helix and irregular loops and on the other, more open side of the domain, by an alpha-helix containing the catalytic tyrosine residue (the catalytic helix). Longer domain constructs extending to amino acids 117 and 124 were also characterized. They contain an additional alpha-helix, are monomeric, and exhibit catalytic activity indistinguishable from that of M-Rep2-95. The binding site for the catalytic metal was identified by paramagnetic broadening and maps to residues on the exposed face of the central beta-sheet. A comparison with the previously determined Rep endonuclease domain structures of tomato yellow leaf curl Sardinia virus (TYLCSV), a geminivirus, and that of porcine circovirus type 2 (PCV2) Rep allows the identification of a positively charged surface that is most likely involved in dsDNA binding, and reveals common features shared by all endonuclease domains of nanovirus, geminivirus, and circovirus Rep proteins.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17472345}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17472345 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17472345}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 2HWT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Faba_bean_necrotic_yellows_virus Faba bean necrotic yellows virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HWT OCA]. | + | 2HWT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Faba_bean_necrotic_yellows_virus Faba bean necrotic yellows virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HWT OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Alpha]] | | [[Category: Alpha]] |
| | [[Category: Beta]] | | [[Category: Beta]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:48:32 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:40:23 2008'' |
Revision as of 10:40, 29 July 2008
Template:STRUCTURE 2hwt
NMR solution structure of the Master-Rep protein nuclease domain (2-95) from the Faba Bean Necrotic Yellows Virus
Template:ABSTRACT PUBMED 17472345
About this Structure
2HWT is a Single protein structure of sequence from Faba bean necrotic yellows virus. Full experimental information is available from OCA.
Reference
Solution structure of the endonuclease domain from the master replication initiator protein of the nanovirus faba bean necrotic yellows virus and comparison with the corresponding geminivirus and circovirus structures., Vega-Rocha S, Gronenborn B, Gronenborn AM, Campos-Olivas R, Biochemistry. 2007 May 29;46(21):6201-12. Epub 2007 May 2. PMID:17472345
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