1ro2

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{{STRUCTURE_1ro2| PDB=1ro2 | SCENE= }}
{{STRUCTURE_1ro2| PDB=1ro2 | SCENE= }}
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'''Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M manganese soak'''
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===Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M manganese soak===
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==Overview==
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Genome replication generally requires primases, which synthesize an initial oligonucleotide primer, and DNA polymerases, which elongate the primer. Primase and DNA polymerase activities are combined, however, in newly identified replicases from archaeal plasmids, such as pRN1 from Sulfolobus islandicus. Here we present a structure-function analysis of the pRN1 primase-polymerase (prim-pol) domain. The crystal structure shows a central depression lined by conserved residues. Mutations on one side of the depression reduce DNA affinity. On the opposite side of the depression cluster three acidic residues and a histidine, which are required for primase and DNA polymerase activity. One acidic residue binds a manganese ion, suggestive of a metal-dependent catalytic mechanism. The structure does not show any similarity to DNA polymerases, but is distantly related to archaeal and eukaryotic primases, with corresponding active-site residues. We propose that archaeal and eukaryotic primases and the prim-pol domain have a common evolutionary ancestor, a bifunctional replicase for small DNA genomes.
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{{ABSTRACT_PUBMED_14730355}}
==About this Structure==
==About this Structure==
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[[Category: Primase]]
[[Category: Primase]]
[[Category: Replication]]
[[Category: Replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:42:46 2008''

Revision as of 10:42, 29 July 2008

Image:1ro2.png

Template:STRUCTURE 1ro2

Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M manganese soak

Template:ABSTRACT PUBMED 14730355

About this Structure

1RO2 is a Single protein structure of sequence from Sulfolobus islandicus. Full crystallographic information is available from OCA.

Reference

Structure of a bifunctional DNA primase-polymerase., Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P, Nat Struct Mol Biol. 2004 Feb;11(2):157-62. Epub 2004 Jan 18. PMID:14730355

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