1zkk

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{{STRUCTURE_1zkk| PDB=1zkk | SCENE= }}
{{STRUCTURE_1zkk| PDB=1zkk | SCENE= }}
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'''Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy'''
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===Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy===
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==Overview==
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SET8 (also known as PR-SET7) is a histone H4-Lys-20-specific methyltransferase that is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. Herein we report the crystal structure of human SET8 (hSET8) bound to a histone H4 peptide bearing Lys-20 and the product cofactor S-adenosylhomocysteine. Histone H4 intercalates in the substrate-binding cleft as an extended parallel beta-strand. Residues preceding Lys-20 in H4 engage in an extensive array of salt bridge, hydrogen bond, and van der Waals interactions with hSET8, while the C-terminal residues bind through predominantly hydrophobic interactions. Mutational analysis of both the substrate-binding cleft and histone H4 reveals that interactions with residues in the N and C termini of the H4 peptide are critical for conferring substrate specificity. Finally, analysis of the product specificity indicates that hSET8 is a monomethylase, consistent with its role in the maintenance of Lys-20 monomethylation during cell division.
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(as it appears on PubMed at http://www.pubmed.gov), where 15933070 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15933070}}
==About this Structure==
==About this Structure==
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[[Category: Histone h4]]
[[Category: Histone h4]]
[[Category: Pseudo-knot]]
[[Category: Pseudo-knot]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:44:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:44:45 2008''

Revision as of 10:44, 29 July 2008

Image:1zkk.png

Template:STRUCTURE 1zkk

Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy

Template:ABSTRACT PUBMED 15933070

About this Structure

1ZKK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of SET8, a histone H4 Lys-20 methyltransferase., Couture JF, Collazo E, Brunzelle JS, Trievel RC, Genes Dev. 2005 Jun 15;19(12):1455-65. Epub 2005 Jun 2. PMID:15933070

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