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2o9r

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[[Image:2o9r.jpg|left|200px]]
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{{STRUCTURE_2o9r| PDB=2o9r | SCENE= }}
{{STRUCTURE_2o9r| PDB=2o9r | SCENE= }}
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'''beta-glucosidase B complexed with thiocellobiose'''
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===beta-glucosidase B complexed with thiocellobiose===
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==Overview==
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Bacteria species involved in degradation of cellulosic substrates produce a variety of enzymes for processing related compounds along the hydrolytic pathway. Paenibacillus polymyxa encodes two homologous beta-glucosidases, BglA and BglB, presenting different quaternary structures and substrate specificities. We previously reported the 3D-structure of BglA, which is highly specific against cellobiose. Here, we present structural analysis of BglB, a monomeric enzyme that acts as an exo-beta-glucosidase hydrolyzing cellobiose and cellodextrins of higher degree of polymerization. The crystal structure of BglB shows that several polar residues narrow the active site pocket and contour additional subsites. The structure of the BglB-cellotetraose complex confirms these subsites, revealing the substrate-binding mode, and shows the oligosaccharide-enzyme recognition pattern in detail. Comparison between BglA and BglB crystal structures suggests that oligomerization in BglA can assist in fine-tuning the specificity of the active centre by modulating the loops surrounding the cavity. We have solved the crystal structure of BglB with bound thiocellobiose, a competitive inhibitor, which together with the BglB-cellotetraose complex delineate the general features of the aglycon site. The detailed characterization of the atomic interactions at the aglycon site show a recognition pattern common to all bacterial beta-glucosidases, and presents some differences with the aglycon site in plant beta-glycosidases essentially by means of a different orientation of the basal Trp. The crystal structures of of BglB with a covalently bound inhibitor (derived from 2-fluoroglucoside) and glucose (produced by hydrolysis of the substrate in the crystal), provide additional pictures of the binding events and the intermediates formed during the reaction. Altogether, this information can assist in the understanding of subtle differences of the enzyme mechanism and substrate recognition within this family of enzymes, and consequently it can help in the development of new enzymes with improved activity or specificity.
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{{ABSTRACT_PUBMED_17585934}}
==About this Structure==
==About this Structure==
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[[Category: Glycosyl hydrolase family 1]]
[[Category: Glycosyl hydrolase family 1]]
[[Category: Thiocellobiose inhibitor]]
[[Category: Thiocellobiose inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:29:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:50:46 2008''

Revision as of 10:50, 29 July 2008

Image:2o9r.png

Template:STRUCTURE 2o9r

beta-glucosidase B complexed with thiocellobiose

Template:ABSTRACT PUBMED 17585934

About this Structure

2O9R is a Single protein structure of sequence from Paenibacillus polymyxa. Full crystallographic information is available from OCA.

Reference

Crystal structures of Paenibacillus polymyxa beta-glucosidase B complexes reveal the molecular basis of substrate specificity and give new insights into the catalytic machinery of family I glycosidases., Isorna P, Polaina J, Latorre-Garcia L, Canada FJ, Gonzalez B, Sanz-Aparicio J, J Mol Biol. 2007 Aug 31;371(5):1204-18. Epub 2007 Jun 2. PMID:17585934

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