1p2y

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{{STRUCTURE_1p2y| PDB=1p2y | SCENE= }}
{{STRUCTURE_1p2y| PDB=1p2y | SCENE= }}
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'''CRYSTAL STRUCTURE OF CYTOCHROME P450CAM IN COMPLEX WITH (S)-(-)-NICOTINE'''
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===CRYSTAL STRUCTURE OF CYTOCHROME P450CAM IN COMPLEX WITH (S)-(-)-NICOTINE===
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==Overview==
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Crystallographic and spectroscopic studies have been undertaken to characterize the binding behavior of the non-native substrate nicotine in the active site of the monooxygenase hemoprotein cytochrome P450cam. Despite the existence of a theoretical model that is consistent with the observed distribution of monooxygenation products, the crystal structure of the complex indicates that the primary binding mode of nicotine is unproductive. The structure is confirmed by spectral data that indicate direct coordination of substrate pyridine nitrogen with the heme iron. This would be the proper structure for evaluating binding affinity and inhibition. Reduction of the heme from Fe(III) to Fe(II) and introduction of carbon monoxide into crystals of the nicotine-P450cam complex, to simulate molecular oxygen binding, produces reorientation of the nicotine. This orientation is the appropriate one for predicting regioselectivity and the kinetic features of substrate oxidation. While it is not clear that such complicated behavior will be exhibited for other enzyme-substrate interactions, it is clear that a single crystal structure for a given substrate-enzyme interaction may not provide a good description of the binding mode responsible for product formation.
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(as it appears on PubMed at http://www.pubmed.gov), where 14556625 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14556625}}
==About this Structure==
==About this Structure==
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[[Category: Nicotine]]
[[Category: Nicotine]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:36:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:53:24 2008''

Revision as of 10:53, 29 July 2008

Image:1p2y.png

Template:STRUCTURE 1p2y

CRYSTAL STRUCTURE OF CYTOCHROME P450CAM IN COMPLEX WITH (S)-(-)-NICOTINE

Template:ABSTRACT PUBMED 14556625

About this Structure

1P2Y is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Crystallographic studies on the complex behavior of nicotine binding to P450cam (CYP101)., Strickler M, Goldstein BM, Maxfield K, Shireman L, Kim G, Matteson DS, Jones JP, Biochemistry. 2003 Oct 21;42(41):11943-50. PMID:14556625

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