2itm

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{{STRUCTURE_2itm| PDB=2itm | SCENE= }}
{{STRUCTURE_2itm| PDB=2itm | SCENE= }}
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'''Crystal structure of the E. coli xylulose kinase complexed with xylulose'''
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===Crystal structure of the E. coli xylulose kinase complexed with xylulose===
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==Overview==
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The primary metabolic route for D-xylose, the second most abundant sugar in nature, is via the pentose phosphate pathway after a two-step or three-step conversion to xylulose-5-phosphate. Xylulose kinase (XK; EC 2.7.1.17) phosphorylates D-xylulose, the last step in this conversion. The apo and D-xylulose-bound crystal structures of Escherichia coli XK have been determined and show a dimer composed of two domains separated by an open cleft. XK dimerization was observed directly by a cryo-EM reconstruction at 36 A resolution. Kinetic studies reveal that XK has a weak substrate-independent MgATP-hydrolyzing activity, and phosphorylates several sugars and polyols with low catalytic efficiency. Binding of pentulose and MgATP to form the reactive ternary complex is strongly synergistic. Although the steady-state kinetic mechanism of XK is formally random, a path is preferred in which D-xylulose binds before MgATP. Modelling of MgATP binding to XK and the accompanying conformational change suggests that sugar binding is accompanied by a dramatic hinge-bending movement that enhances interactions with MgATP, explaining the observed synergism. A catalytic mechanism is proposed and supported by relevant site-directed mutants.
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(as it appears on PubMed at http://www.pubmed.gov), where 17123542 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17123542}}
==About this Structure==
==About this Structure==
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[[Category: Xylulokinase]]
[[Category: Xylulokinase]]
[[Category: Xylulose]]
[[Category: Xylulose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:51:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:05:52 2008''

Revision as of 11:06, 29 July 2008

Image:2itm.png

Template:STRUCTURE 2itm

Crystal structure of the E. coli xylulose kinase complexed with xylulose

Template:ABSTRACT PUBMED 17123542

About this Structure

2ITM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli., Di Luccio E, Petschacher B, Voegtli J, Chou HT, Stahlberg H, Nidetzky B, Wilson DK, J Mol Biol. 2007 Jan 19;365(3):783-98. Epub 2006 Oct 25. PMID:17123542

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