1nat

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{{STRUCTURE_1nat| PDB=1nat | SCENE= }}
{{STRUCTURE_1nat| PDB=1nat | SCENE= }}
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'''CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS'''
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===CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS===
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==Overview==
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Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound in the active site. The crystal structure of wild-type Spo0F in the absence of a metal ion is presented here. A comparison of the two structures reveals that the cation induces significant changes in the active site. In the present wild-type structure, the carboxylate of Asp11 points away from the center of the active site, whereas when coordinated to the Ca2+, as in the earlier structure, it points toward the active site. In addition, Asp54, the site of phosphorylation, is blocked by a salt bridge interaction of an Arg side chain from a neighboring molecule. From fluorescence quenching studies with Spo0F Y13W, we found that only the amino acid Arg binds to Spo0F in a saturable manner (Kd = 15 mM). This observation suggests that a small molecule with a shape complementary to the active site and having a guanidinium group might inhibit phosphotransfer between response regulators and their cognate histidine kinases.
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(as it appears on PubMed at http://www.pubmed.gov), where 9335530 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9335530}}
==About this Structure==
==About this Structure==
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[[Category: Sporulation response regulator]]
[[Category: Sporulation response regulator]]
[[Category: Two component system]]
[[Category: Two component system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:18:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:09:26 2008''

Revision as of 11:09, 29 July 2008

Image:1nat.png

Template:STRUCTURE 1nat

CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS

Template:ABSTRACT PUBMED 9335530

About this Structure

1NAT is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:9335530

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