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| - | [[Image:2owm.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2owm| PDB=2owm | SCENE= }} | | {{STRUCTURE_2owm| PDB=2owm | SCENE= }} |
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| - | '''Motor domain of Neurospora crassa kinesin-3 (NcKin3)'''
| + | ===Motor domain of Neurospora crassa kinesin-3 (NcKin3)=== |
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| - | ==Overview==
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| - | Neurospora crassa kinesin NcKin3 belongs to a unique fungal-specific subgroup of small Kinesin-3-related motor proteins. One of its functions appears to be the transport of mitochondria along microtubules. Here, we present the X-ray structure of a C-terminally truncated monomeric construct of NcKin3 comprising the motor domain and the neck linker, and a 3-D image reconstruction of this motor domain bound to microtubules, by cryoelectron microscopy. The protein contains Mg.ADP bound to the active site, yet the structure resembles an ATP-bound state. By comparison with structures of the Kinesin-3 motor Kif1A in different nucleotide states (Kikkawa, M. et al. (2001) Nature (London, U.K.) 411, 439-445), the NcKin3 structure corresponds to the AMPPCP complex of Kif1A rather than the AMPPNP complex. NcKin3-specific differences in the coordination of the nucleotide and asymmetric interactions between adjacent molecules in the crystal are discussed in the context of the unusual kinetics of the dimeric wild-type motor and the monomeric construct used for crystal structure analysis. The NcKin3 motor decorates microtubules at a stoichiometry of one head per alphabeta-tubulin heterodimer, thereby forming an axial periodicity of 8 nm. In spite of unusual extensions at the N-terminus and within flexible loops L2, L8a, and L12 (corresponding to the K-loop of monomeric kinesins), the microtubule binding geometry is similar to that of other members of the kinesin family.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_18205396}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18205396 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_18205396}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Motor protein]] | | [[Category: Motor protein]] |
| | [[Category: Neck linker]] | | [[Category: Neck linker]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:49:06 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:13:32 2008'' |
Revision as of 11:13, 29 July 2008
Template:STRUCTURE 2owm
Motor domain of Neurospora crassa kinesin-3 (NcKin3)
Template:ABSTRACT PUBMED 18205396
About this Structure
2OWM is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
Reference
X-ray Structure and Microtubule Interaction of the Motor Domain of Neurospora crassa NcKin3, a Kinesin with Unusual Processivity(,)., Marx A, Muller J, Mandelkow EM, Woehlke G, Bouchet-Marquis C, Hoenger A, Mandelkow E, Biochemistry. 2008 Feb 19;47(7):1848-1861. Epub 2008 Jan 19. PMID:18205396
Page seeded by OCA on Tue Jul 29 14:13:32 2008
