This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1rxz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1rxz.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1rxz.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1rxz| PDB=1rxz | SCENE= }}
{{STRUCTURE_1rxz| PDB=1rxz | SCENE= }}
-
'''C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA'''
+
===C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA===
-
==Overview==
+
<!--
-
Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14718165}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14718165 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14718165}}
==About this Structure==
==About this Structure==
Line 33: Line 37:
[[Category: Dna replication]]
[[Category: Dna replication]]
[[Category: Interdomain connecting loop]]
[[Category: Interdomain connecting loop]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:02:42 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:14:01 2008''

Revision as of 11:14, 29 July 2008

Image:1rxz.png

Template:STRUCTURE 1rxz

C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA

Template:ABSTRACT PUBMED 14718165

About this Structure

1RXZ is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165

Page seeded by OCA on Tue Jul 29 14:14:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rxz"
Personal tools