1u7c

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{{STRUCTURE_1u7c| PDB=1u7c | SCENE= }}
{{STRUCTURE_1u7c| PDB=1u7c | SCENE= }}
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'''Crystal Structure of AmtB from E.Coli with Methyl Ammonium.'''
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===Crystal Structure of AmtB from E.Coli with Methyl Ammonium.===
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==Overview==
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The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.
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(as it appears on PubMed at http://www.pubmed.gov), where 15361618 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15361618}}
==About this Structure==
==About this Structure==
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[[Category: Right handed helical bundle]]
[[Category: Right handed helical bundle]]
[[Category: Transmembrane helice]]
[[Category: Transmembrane helice]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:50:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:14:39 2008''

Revision as of 11:14, 29 July 2008

Image:1u7c.png

Template:STRUCTURE 1u7c

Crystal Structure of AmtB from E.Coli with Methyl Ammonium.

Template:ABSTRACT PUBMED 15361618

About this Structure

1U7C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:15361618

Page seeded by OCA on Tue Jul 29 14:14:39 2008

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