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| | {{STRUCTURE_1rfs| PDB=1rfs | SCENE= }} | | {{STRUCTURE_1rfs| PDB=1rfs | SCENE= }} |
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| - | '''RIESKE SOLUBLE FRAGMENT FROM SPINACH'''
| + | ===RIESKE SOLUBLE FRAGMENT FROM SPINACH=== |
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| - | ==Overview==
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| - | BACKGROUND: The cytochrome b6f complex functions in oxygenic photosynthesis as an integral membrane protein complex that mediates coupled electron transfer and proton translocation. The Rieske [2Fe-2S] protein subunit of the complex functions at the electropositive (p) membrane interface as the electron acceptor for plastoquinol and donor for the cytochrome f subunit, and may have a dynamic role in catalyzing electron and proton transfer at the membrane interface. There are significant structure/function similarities to the cytochrome bc1 complex of the respiratory chain. RESULTS: The 1.83 A crystal structure of a 139-residue C-terminal fragment of the Rieske [2Fe-2S] protein, derived from the cytochrome b6f complex of spinach chloroplasts, has been solved by multiwavelength anomalous diffraction. The structure of the fragment comprises two domains: a small 'cluster-binding' subdomain and a large subdomain. The [2Fe-2S] cluster-binding subdomains of the chloroplast and mitochondrial Rieske proteins are virtually identical, whereas the large subdomains are strikingly different despite a common folding topology. A structure-based sequence alignment of the b6f and bc1 groups of Rieske soluble domains is presented. CONCLUSIONS: The segregation of structural conservation and divergence in the cluster-binding and large subdomains of the Rieske protein correlates with the overall relatedness of the cytochrome b6f and bc1 complexes, in which redox domains in the aqueous p phase are dissimilar and those within the membrane are similar. Distinct sequences and surface charge distributions among Rieske large subdomains may provide a signature for interaction with the p-side oxidant protein and for the pH of the intraorganelle compartment.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9438861}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9438861 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9438861}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Electron transport]] | | [[Category: Electron transport]] |
| | [[Category: Iron-sulfur protein]] | | [[Category: Iron-sulfur protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:26:45 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:17:08 2008'' |
Revision as of 11:17, 29 July 2008
Template:STRUCTURE 1rfs
RIESKE SOLUBLE FRAGMENT FROM SPINACH
Template:ABSTRACT PUBMED 9438861
About this Structure
1RFS is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein., Carrell CJ, Zhang H, Cramer WA, Smith JL, Structure. 1997 Dec 15;5(12):1613-25. PMID:9438861
Page seeded by OCA on Tue Jul 29 14:17:08 2008
