2i26

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{{STRUCTURE_2i26| PDB=2i26 | SCENE= }}
{{STRUCTURE_2i26| PDB=2i26 | SCENE= }}
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'''Crystal structure analysis of the nurse shark new antigen receptor ancestral variable domain in complex with lysozyme'''
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===Crystal structure analysis of the nurse shark new antigen receptor ancestral variable domain in complex with lysozyme===
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==Overview==
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Sharks express an unusual heavy-chain isotype called IgNAR, whose variable regions bind antigen as independent soluble domains. To further probe affinity maturation of the IgNAR response, we structurally characterized the germline and somatically matured versions of a type II variable (V) region, both in the presence and absence of its antigen, hen egg-white lysozyme. Despite a disulfide bond linking complementarity determining regions (CDRs) 1 and 3, both germline and somatically matured V regions displayed significant structural changes in these CDRs upon complex formation with antigen. Somatic mutations in the IgNAR V region serve to increase the number of contacts with antigen, as reflected by a tenfold increase in affinity, and one of these mutations appears to stabilize the CDR3 region. In addition, a residue in the HV4 loop plays an important role in antibody-antigen interaction, consistent with the high rate of somatic mutations in this non-CDR loop.
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(as it appears on PubMed at http://www.pubmed.gov), where 17258766 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17258766}}
==About this Structure==
==About this Structure==
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[[Category: Immunoglobulin fold]]
[[Category: Immunoglobulin fold]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:59:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:17:22 2008''

Revision as of 11:17, 29 July 2008

Image:2i26.png

Template:STRUCTURE 2i26

Crystal structure analysis of the nurse shark new antigen receptor ancestral variable domain in complex with lysozyme

Template:ABSTRACT PUBMED 17258766

About this Structure

2I26 is a Single protein structure of sequence from Gallus gallus and Ginglymostoma cirratum. Full crystallographic information is available from OCA.

Reference

Maturation of shark single-domain (IgNAR) antibodies: evidence for induced-fit binding., Stanfield RL, Dooley H, Verdino P, Flajnik MF, Wilson IA, J Mol Biol. 2007 Mar 23;367(2):358-72. Epub 2006 Dec 22. PMID:17258766

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