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2h6e

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{{STRUCTURE_2h6e| PDB=2h6e | SCENE= }}
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'''Crystal structure of the D-arabinose dehydrogenase from Sulfolobus solfataricus'''
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===Crystal structure of the D-arabinose dehydrogenase from Sulfolobus solfataricus===
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==Overview==
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Sulfolobus solfataricus metabolizes the five-carbon sugar d-arabinose to 2-oxoglutarate by an inducible pathway consisting of dehydrogenases and dehydratases. Here we report the crystal structure and biochemical properties of the first enzyme of this pathway: the d-arabinose dehydrogenase. The AraDH structure was solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure revealed a catalytic and cofactor binding domain, typically present in mesophilic and thermophilic alcohol dehydrogenases. Cofactor modeling showed the presence of a phosphate binding pocket sequence motif (SRS-X2-H), which is likely to be responsible for the enzyme's preference for NADP+. The homo-tetrameric enzyme is specific for d-arabinose, l-fucose, l-galactose and d-ribose, which could be explained by the hydrogen bonding patterns of the C3 and C4 hydroxyl groups observed in substrate docking simulations. The enzyme optimally converts sugars at pH 8.2 and 91 degrees C, and displays a half-life of 42 and 26 min at 85 and 90 degrees C, respectively, indicating that the enzyme is thermostable at physiological operating temperatures of 80 degrees C. The structure represents the first crystal structure of an NADP+-dependent member of the medium-chain dehydrogenase/reductase (MDR) superfamily from Archaea.
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==About this Structure==
==About this Structure==
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[[Category: Medium chain alcohol dehydrogenase]]
[[Category: Medium chain alcohol dehydrogenase]]
[[Category: Rossman fold]]
[[Category: Rossman fold]]
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Revision as of 11:20, 29 July 2008

Image:2h6e.png

Template:STRUCTURE 2h6e

Crystal structure of the D-arabinose dehydrogenase from Sulfolobus solfataricus

Template:ABSTRACT PUBMED 17610898

About this Structure

2H6E is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Crystal structure and biochemical properties of the D-arabinose dehydrogenase from Sulfolobus solfataricus., Brouns SJ, Turnbull AP, Willemen HL, Akerboom J, van der Oost J, J Mol Biol. 2007 Aug 31;371(5):1249-60. Epub 2007 Jun 8. PMID:17610898

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