2hem

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[[Image:2hem.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2hem| PDB=2hem | SCENE= }}
{{STRUCTURE_2hem| PDB=2hem | SCENE= }}
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'''NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.'''
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===NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.===
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==Overview==
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Helix 42 of Domain II of Escherichia coli 23S ribosomal RNA underlies the L7/L12 stalk in the ribosome and may be significant in positioning this feature relative to the rest of the 50S ribosomal subunit. Unlike the Haloarcula marismortui and Deinococcus radiodurans examples, the lower portion of helix 42 in E.coli contains two consecutive G*A oppositions with both adenines on the same side of the stem. Herein, the structure of an analog of positions 1037-1043 and 1112-1118 in the helix 42 region is reported. NMR spectra and structure calculations support a cis Watson-Crick/Watson-Crick (cis W.C.) G*A conformation for the tandem (G*A)2 in the analog and a minimally perturbed helical duplex stem. Mg2+ titration studies imply that the cis W.C. geometry of the tandem (G*A)2 probably allows O6 of G20 and N1 of A4 to coordinate with a Mg2+ ion as indicated by the largest chemical shift changes associated with the imino group of G20 and the H8 of G20 and A4. A cross-strand bridging Mg2+ coordination has also been found in a different sequence context in the crystal structure of H.marismortui 23S rRNA, and therefore it may be a rare but general motif in Mg2+ coordination.
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(as it appears on PubMed at http://www.pubmed.gov), where 15939932 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15939932}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEM OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEM OCA].
==Reference==
==Reference==
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[[Category: L7/l12 stalk]]
[[Category: L7/l12 stalk]]
[[Category: Mg2+ binding]]
[[Category: Mg2+ binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:11:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:41:29 2008''

Revision as of 11:41, 29 July 2008

Image:2hem.png

Template:STRUCTURE 2hem

NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit.

Template:ABSTRACT PUBMED 15939932

About this Structure

Full experimental information is available from OCA.

Reference

NMR structure and Mg2+ binding of an RNA segment that underlies the L7/L12 stalk in the E.coli 50S ribosomal subunit., Zhao Q, Nagaswamy U, Lee H, Xia Y, Huang HC, Gao X, Fox GE, Nucleic Acids Res. 2005 Jun 6;33(10):3145-53. Print 2005. PMID:15939932

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