2eqb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2eqb.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2eqb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2eqb| PDB=2eqb | SCENE= }}
{{STRUCTURE_2eqb| PDB=2eqb | SCENE= }}
-
'''Crystal structure of the Rab GTPase Sec4p, the Sec2p GEF domain, and phosphate complex'''
+
===Crystal structure of the Rab GTPase Sec4p, the Sec2p GEF domain, and phosphate complex===
-
==Overview==
+
<!--
-
Vesicular transport during exocytosis is regulated by Rab GTPase (Sec4p in yeast), which is activated by a guanine nucleotide exchange factor (GEF) called Sec2p. Here, we report the crystal structure of the Sec2p GEF domain in a complex with the nucleotide-free Sec4p at 2.7 A resolution. Upon complex formation, the Sec2p helices approach each other, flipping the side chain of Phe-109 toward Leu-104 and Leu-108 of Sec2p. These three residues provide a hydrophobic platform to attract the side chains of Phe-49, Ile-53, and Ile-55 in the switch I region as well as Phe-57 and Trp-74 in the interswitch region of Sec4p. Consequently, the switch I and II regions are largely deformed, to create a flat hydrophobic interface that snugly fits the surface of the Sec2p coiled coil. These drastic conformational changes disrupt the interactions between switch I and the bound guanine nucleotide, which facilitates the GDP release. Unlike the recently reported 3.3 A structure of the Sec4p.Sec2p complex, our structure contains a phosphate ion bound to the P-loop, which may represent an intermediate state of the nucleotide exchange reaction.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17488829}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17488829 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17488829}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Sato, Y.]]
[[Category: Sato, Y.]]
[[Category: Coiled coil]]
[[Category: Coiled coil]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:59:23 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:44:05 2008''

Revision as of 11:44, 29 July 2008

Image:2eqb.png

Template:STRUCTURE 2eqb

Crystal structure of the Rab GTPase Sec4p, the Sec2p GEF domain, and phosphate complex

Template:ABSTRACT PUBMED 17488829

About this Structure

2EQB is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state., Sato Y, Fukai S, Ishitani R, Nureki O, Proc Natl Acad Sci U S A. 2007 May 15;104(20):8305-10. Epub 2007 May 8. PMID:17488829

Page seeded by OCA on Tue Jul 29 14:44:05 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2eqb"
Personal tools