2oih

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[[Image:2oih.gif|left|200px]]
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{{Seed}}
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[[Image:2oih.png|left|200px]]
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{{STRUCTURE_2oih| PDB=2oih | SCENE= }}
{{STRUCTURE_2oih| PDB=2oih | SCENE= }}
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'''Hepatitis Delta Virus gemonic ribozyme precursor with C75U mutation and bound to monovalent cation Tl+'''
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===Hepatitis Delta Virus gemonic ribozyme precursor with C75U mutation and bound to monovalent cation Tl+===
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==Overview==
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The hepatitis delta virus (HDV) ribozyme catalyzes viral RNA self-cleavage through general acid-base chemistry in which an active-site cytidine and at least one metal ion are involved. Monovalent metal ions support slow catalysis and were proposed to substitute for structural, but not catalytic, divalent metal ions in the RNA. To investigate the role of monovalent cations in ribozyme structure and function, we determined the crystal structure of the precursor HDV ribozyme in the presence of thallium ions (Tl(+)). Two Tl(+) ions can occupy a previously observed divalent metal ion hexahydrate-binding site located near the scissile phosphate, but are easily competed away by cobalt hexammine, a magnesium hexahydrate mimic and potent reaction inhibitor. Intriguingly, a third Tl(+) ion forms direct inner-sphere contacts with the ribose 2'-OH nucleophile and the pro-S(p) scissile phosphate oxygen. We discuss possible structural and catalytic implications of monovalent cation binding for the HDV ribozyme mechanism.
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The line below this paragraph, {{ABSTRACT_PUBMED_17355864}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17355864 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17355864}}
==About this Structure==
==About this Structure==
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[[Category: Ke, A.]]
[[Category: Ke, A.]]
[[Category: Tl+-bound]]
[[Category: Tl+-bound]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:59:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:47:33 2008''

Revision as of 11:47, 29 July 2008

Image:2oih.png

Template:STRUCTURE 2oih

Hepatitis Delta Virus gemonic ribozyme precursor with C75U mutation and bound to monovalent cation Tl+

Template:ABSTRACT PUBMED 17355864

About this Structure

2OIH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural roles of monovalent cations in the HDV ribozyme., Ke A, Ding F, Batchelor JD, Doudna JA, Structure. 2007 Mar;15(3):281-7. PMID:17355864

Page seeded by OCA on Tue Jul 29 14:47:33 2008

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