2pyu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2pyu.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2pyu.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2pyu| PDB=2pyu | SCENE= }}
{{STRUCTURE_2pyu| PDB=2pyu | SCENE= }}
-
'''Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with IMP'''
+
===Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with IMP===
-
==Overview==
+
<!--
-
Inosine triphosphate pyrophosphatases, which are ubiquitous house-cleaning enzymes, hydrolyze noncanonical nucleoside triphosphates (inosine triphosphate (ITP) and xanthosine triphosphate (XTP)) and prevent the incorporation of hypoxanthine or xanthine into nascent DNA or RNA. Here we present the 1.5-A-resolution crystal structure of the inosine triphosphate pyrophosphatase RdgB from Escherichia coli in a free state and in complex with a substrate (ITP+Ca(2+)) or a product (inosine monophosphate (IMP)). ITP binding to RdgB induced a large displacement of the alpha1 helix, closing the enzyme active site. This positions the conserved Lys13 close to the bridging oxygen between the alpha- and beta-phosphates of the substrate, weakening the P(alpha)-O bond. On the other side of the substrate, the conserved Asp69 is proposed to act as a base coordinating the catalytic water molecule. Our data provide insight into the molecular mechanisms of the substrate selectivity and catalysis of RdgB and other ITPases.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17976651}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17976651 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17976651}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Itp pyrophosphatase x-ray structure enzyme mechanism product]]
[[Category: Itp pyrophosphatase x-ray structure enzyme mechanism product]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:02:15 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:49:49 2008''

Revision as of 11:49, 29 July 2008

Image:2pyu.png

Template:STRUCTURE 2pyu

Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with IMP

Template:ABSTRACT PUBMED 17976651

About this Structure

2PYU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Molecular basis of the antimutagenic activity of the house-cleaning inosine triphosphate pyrophosphatase RdgB from Escherichia coli., Savchenko A, Proudfoot M, Skarina T, Singer A, Litvinova O, Sanishvili R, Brown G, Chirgadze N, Yakunin AF, J Mol Biol. 2007 Dec 7;374(4):1091-103. Epub 2007 Oct 11. PMID:17976651

Page seeded by OCA on Tue Jul 29 14:49:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pyu"
Personal tools