2i65

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[[Image:2i65.gif|left|200px]]
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{{STRUCTURE_2i65| PDB=2i65 | SCENE= }}
{{STRUCTURE_2i65| PDB=2i65 | SCENE= }}
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'''Structural Basis for the Mechanistic Understanding Human CD38 Controlled Multiple Catalysis'''
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===Structural Basis for the Mechanistic Understanding Human CD38 Controlled Multiple Catalysis===
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==Overview==
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The enzymatic cleavage of the nicotinamide-glycosidic bond on nicotinamide adenine dinucleotide (NAD(+)) has been proposed to go through an oxocarbenium ion-like transition state. Because of the instability of the ionic intermediate, there has been no structural report on such a transient reactive species. Human CD38 is an ectoenzyme that can use NAD(+) to synthesize two calcium-mobilizing molecules. By using NAD(+) and a surrogate substrate, NGD(+), we captured and determined crystal structures of the enzyme complexed with an intermediate, a substrate, and a product along the reaction pathway. Our results showed that the intermediate is stabilized by polar interactions with the catalytic residue Glu(226) rather than by a covalent linkage. The polar interactions between Glu(226) and the substrate 2',3'-OH groups are essential for initiating catalysis. Ser(193) was demonstrated to have a regulative role during catalysis and is likely to be involved in intermediate stabilization. In addition, a product inhibition effect by ADP-ribose (through the reorientation of the product) or GDP-ribose (through the formation of a covalently linked GDP-ribose dimer) was observed. These structural data provide insights into the understanding of multiple catalysis and clues for drug design.
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(as it appears on PubMed at http://www.pubmed.gov), where 16951430 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16951430}}
==About this Structure==
==About this Structure==
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[[Category: Reaction product]]
[[Category: Reaction product]]
[[Category: The catalytic pocket]]
[[Category: The catalytic pocket]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:07:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:01:20 2008''

Revision as of 12:01, 29 July 2008

Image:2i65.png

Template:STRUCTURE 2i65

Structural Basis for the Mechanistic Understanding Human CD38 Controlled Multiple Catalysis

Template:ABSTRACT PUBMED 16951430

About this Structure

2I65 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for the mechanistic understanding of human CD38-controlled multiple catalysis., Liu Q, Kriksunov IA, Graeff R, Munshi C, Lee HC, Hao Q, J Biol Chem. 2006 Oct 27;281(43):32861-9. Epub 2006 Sep 2. PMID:16951430

Page seeded by OCA on Tue Jul 29 15:01:20 2008

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