2cbf

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{{STRUCTURE_2cbf| PDB=2cbf | SCENE= }}
{{STRUCTURE_2cbf| PDB=2cbf | SCENE= }}
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'''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF'''
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===THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF===
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==Overview==
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Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.
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(as it appears on PubMed at http://www.pubmed.gov), where 9665173 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9665173}}
==About this Structure==
==About this Structure==
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[[Category: Methylase]]
[[Category: Methylase]]
[[Category: Precorrin-4 methyltransferase]]
[[Category: Precorrin-4 methyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:39:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:03:45 2008''

Revision as of 12:03, 29 July 2008

Image:2cbf.png

Template:STRUCTURE 2cbf

THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF

Template:ABSTRACT PUBMED 9665173

About this Structure

2CBF is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.

Reference

The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173

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