1qfz

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Revision as of 22:32, 20 November 2007

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spinqualitylabelsall models 1qfz, resolution 1.7Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PEA FNR Y308S MUTANT IN COMPLEX WITH NADPH

Overview

The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production, of NADPH during photosynthesis. Whereas the structures of FNRs from, spinach leaf and a cyanobacterium as well as many of their homologs have, been solved, none of these studies has yielded a productive geometry of, the flavin-nicotinamide interaction. Here, we show that this failure, occurs because nicotinamide binding to wild type FNR involves the, energetically unfavorable displacement of the C-terminal Tyr side chain., We used mutants of this residue (Tyr 308) of pea FNR to obtain the, structures of productive NADP+ and NADPH complexes. These structures, reveal a unique NADP+ binding mode in which the nicotinamide ring is not, parallel to the flavin isoalloxazine ring, but lies against it at an angle, of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom.

About this Structure

1QFZ is a Single protein structure of sequence from Pisum sativum with SO4, FAD and NDP as ligands. Active as Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 Full crystallographic information is available from OCA.

Reference

A productive NADP+ binding mode of ferredoxin-NADP + reductase revealed by protein engineering and crystallographic studies., Deng Z, Aliverti A, Zanetti G, Arakaki AK, Ottado J, Orellano EG, Calcaterra NB, Ceccarelli EA, Carrillo N, Karplus PA, Nat Struct Biol. 1999 Sep;6(9):847-53. PMID:10467097

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