3bam

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3bam.gif|left|200px]]
+
{{Seed}}
 +
[[Image:3bam.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3bam| PDB=3bam | SCENE= }}
{{STRUCTURE_3bam| PDB=3bam | SCENE= }}
-
'''RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND MANGANESE IONS (POST-REACTIVE COMPLEX)'''
+
===RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND MANGANESE IONS (POST-REACTIVE COMPLEX)===
-
==Overview==
+
<!--
-
Type II restriction enzymes are characterized by their remarkable specificity and simplicity. They require only divalent metals (such as Mg2+ or Mn2+) as cofactors to catalyze the hydrolysis of DNA. However, most of the structural work on endonucleases has been performed in the absence of metals, leaving unanswered questions about their mechanisms of DNA cleavage. Here we report structures of the endonuclease BamHI-DNA complex, determined in the presence of Mn2+ and Ca2+, that describe the enzyme at different stages of catalysis. Overall, the results support a two-metal mechanism of DNA cleavage for BamHI which is distinct from that of EcoRV.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9783752}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9783752 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9783752}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Phosphodiesterase]]
[[Category: Phosphodiesterase]]
[[Category: Protein/dna]]
[[Category: Protein/dna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:34:48 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:12:39 2008''

Revision as of 12:12, 29 July 2008

Image:3bam.png

Template:STRUCTURE 3bam

RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND MANGANESE IONS (POST-REACTIVE COMPLEX)

Template:ABSTRACT PUBMED 9783752

About this Structure

3BAM is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

The role of metals in catalysis by the restriction endonuclease BamHI., Viadiu H, Aggarwal AK, Nat Struct Biol. 1998 Oct;5(10):910-6. PMID:9783752

Page seeded by OCA on Tue Jul 29 15:12:39 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bam"
Personal tools