2ohx

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{{STRUCTURE_2ohx| PDB=2ohx | SCENE= }}
{{STRUCTURE_2ohx| PDB=2ohx | SCENE= }}
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'''REFINED CRYSTAL STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE-NADH COMPLEX AT 1.8 ANGSTROMS RESOLUTION'''
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===REFINED CRYSTAL STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE-NADH COMPLEX AT 1.8 ANGSTROMS RESOLUTION===
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==Overview==
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The crystal structure of the ternary complex of horse liver alcohol dehydrogenase (LADH) with the coenzyme NADH and inhibitor dimethyl sulfoxide (DMSO) has been refined by simulated annealing with molecular dynamics and restrained positional refinement using the program X-PLOR. The two subunits of the enzyme were refined independently. The space group was P1 with cell dimensions a = 51.8, b = 44.5, c = 94.6 A, alpha = 104.8, beta = 102.3 and gamma = 70.6 degrees. The resulting crystallographic R factor is 17.3% for 62 440 unique reflections in the resolution range 10.0-1.8 A. A total of 472 ordered solvent molecules were localized in the structure. An analysis of secondary-structure elements, solvent content and NADH binding is presented.
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The line below this paragraph, {{ABSTRACT_PUBMED_15299346}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15299346 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299346}}
==About this Structure==
==About this Structure==
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[[Category: Al-Karadaghi, S.]]
[[Category: Al-Karadaghi, S.]]
[[Category: Cedergren-Zeppezauer, E S.]]
[[Category: Cedergren-Zeppezauer, E S.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:57:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:14:40 2008''

Revision as of 12:14, 29 July 2008

Image:2ohx.png

Template:STRUCTURE 2ohx

REFINED CRYSTAL STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE-NADH COMPLEX AT 1.8 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 15299346

About this Structure

2OHX is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

Reference

Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 A resolution., Al-Karadaghi S, Cedergren-Zeppezauer ES, Hovmoller S, Acta Crystallogr D Biol Crystallogr. 1994 Nov 1;50(Pt 6):793-807. PMID:15299346

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