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2dcz

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[[Image:2dcz.gif|left|200px]]
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{{STRUCTURE_2dcz| PDB=2dcz | SCENE= }}
{{STRUCTURE_2dcz| PDB=2dcz | SCENE= }}
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'''Thermal Stabilization of Bacillus subtilis Family-11 Xylanase By Directed Evolution'''
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===Thermal Stabilization of Bacillus subtilis Family-11 Xylanase By Directed Evolution===
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==Overview==
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We used directed evolution to enhance the thermostability of glycosyl hydrolase family-11 xylanase from Bacillus subtilis. By combining random point mutagenesis, saturation mutagenesis, and DNA shuffling, a thermostable variant, Xyl(st), was identified which contained three amino acid substitutions: Q7H, N8F, and S179C. The half-inactivation temperature (the midpoint of the melting curves) for the Xyl(st) variant compared with the wild-type enzyme after incubation for 10 min was elevated from 58 to 68 degrees C. At 60 degrees C the wild-type enzyme was inactivated within 5 min, but Xyl(st) retained full activity for at least 2 h. The stabilization was accompanied by evidence of thermophilicity; that is, an increase in the optimal reaction temperature from 55 to 65 degrees C and lower activity at low temperatures and higher activity at higher temperatures relative to wild type. To elucidate the mechanism of thermal stabilization, three-dimensional structures were determined for the wild-type and Xyl(st) enzymes. A cavity was identified around Gln-7/Asn-8 in wild type that was filled with bulky, hydrophobic residues in Xyl(st). This site was not identified by previous approaches, but directed evolution identified the region as a weak point. Formation of an intermolecular disulfide bridge via Cys-179 was observed between monomers in Xyl(st). However, the stability was essentially the same in the presence and absence of a reducing agent, indicating that the increased hydrophobicity around the Cys-179 accounted for the stability.
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(as it appears on PubMed at http://www.pubmed.gov), where 16467302 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16467302}}
==About this Structure==
==About this Structure==
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[[Category: Tsuda, S.]]
[[Category: Tsuda, S.]]
[[Category: All beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:11:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:16:39 2008''

Revision as of 12:16, 29 July 2008

Image:2dcz.png

Template:STRUCTURE 2dcz

Thermal Stabilization of Bacillus subtilis Family-11 Xylanase By Directed Evolution

Template:ABSTRACT PUBMED 16467302

About this Structure

2DCZ is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution., Miyazaki K, Takenouchi M, Kondo H, Noro N, Suzuki M, Tsuda S, J Biol Chem. 2006 Apr 14;281(15):10236-42. Epub 2006 Feb 8. PMID:16467302

Page seeded by OCA on Tue Jul 29 15:16:39 2008

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