1pkv

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{{STRUCTURE_1pkv| PDB=1pkv | SCENE= }}
{{STRUCTURE_1pkv| PDB=1pkv | SCENE= }}
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'''The N-terminal domain of riboflavin synthase in complex with riboflavin'''
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===The N-terminal domain of riboflavin synthase in complex with riboflavin===
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==Overview==
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Riboflavin synthase of Escherichia coli is a homotrimer with a molecular mass of 70 kDa. The enzyme catalyzes the dismutation of 6,7-dimethyl-8-(1'-D-ribityl)-lumazine, affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. The N-terminal segment (residues 1-87) and the C-terminal segment (residues 98-187) form beta-barrels with similar fold and a high degree of sequence similarity. A recombinant peptide comprising amino acid residues 1-97 forms a dimer, which binds riboflavin with high affinity. Here, we report the structure of this construct in complex with riboflavin at 2.6A resolution. It is demonstrated that the complex can serve as a model for ligand-binding in the native enzyme. The structure and riboflavin-binding mode is in excellent agreement with structural information obtained from the native enzyme from Escherichia coli and riboflavin synthase from Schizosaccharomyces pombe. The implications for the binding specificity and the regiospecificity of the catalyzed reaction are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 12927541 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12927541}}
==About this Structure==
==About this Structure==
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[[Category: Dimer]]
[[Category: Dimer]]
[[Category: Greek key motif]]
[[Category: Greek key motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:12:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:18:36 2008''

Revision as of 12:18, 29 July 2008

Image:1pkv.png

Template:STRUCTURE 1pkv

The N-terminal domain of riboflavin synthase in complex with riboflavin

Template:ABSTRACT PUBMED 12927541

About this Structure

1PKV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution., Meining W, Eberhardt S, Bacher A, Ladenstein R, J Mol Biol. 2003 Aug 29;331(5):1053-63. PMID:12927541

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