1unc

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[[Image:1unc.gif|left|200px]]
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{{Seed}}
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[[Image:1unc.png|left|200px]]
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{{STRUCTURE_1unc| PDB=1unc | SCENE= }}
{{STRUCTURE_1unc| PDB=1unc | SCENE= }}
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'''SOLUTION STRUCTURE OF THE HUMAN VILLIN C-TERMINAL HEADPIECE SUBDOMAIN'''
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===SOLUTION STRUCTURE OF THE HUMAN VILLIN C-TERMINAL HEADPIECE SUBDOMAIN===
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==Overview==
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Headpiece (HP) is a 76-residue F-actin-binding module at the C terminus of many cytoskeletal proteins. Its 35-residue C-terminal subdomain is one of the smallest known motifs capable of autonomously adopting a stable, folded structure in the absence of any disulfide bridges, metal ligands, or unnatural amino acids. We report the three-dimensional solution structures of the C-terminal headpiece subdomains of human villin (HVcHP) and human advillin (HAcHP), determined by two-dimensional 1H-NMR. They represent the second and third structures of such C-terminal headpiece subdomains to be elucidated so far. A comparison with the structure of the chicken villin C-terminal subdomain reveals a high structural conservation. Both C-terminal subdomains bind specifically to F-actin. Mutagenesis is used to demonstrate the involvement of Trp 64 in the F-actin-binding surface. The latter residue is part of a conserved structural feature, in which the surface-exposed indole ring is stacked on the proline and lysine side chain embedded in a PXWK sequence motif. On the basis of the structural and mutational data concerning Trp 64 reported here, the results of a cysteine-scanning mutagenesis study of full headpiece, and a phage display mutational study of the 69-74 fragment, we propose a modification of the model, elaborated by Vardar and coworkers, for the binding of headpiece to F-actin.
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The line below this paragraph, {{ABSTRACT_PUBMED_15096633}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15096633 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15096633}}
==About this Structure==
==About this Structure==
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1UNC is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UNC OCA].
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1UNC is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UNC OCA].
==Reference==
==Reference==
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[[Category: F-actin binding]]
[[Category: F-actin binding]]
[[Category: Headpiece subdomain]]
[[Category: Headpiece subdomain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:27:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:22:02 2008''

Revision as of 12:22, 29 July 2008

Image:1unc.png

Template:STRUCTURE 1unc

SOLUTION STRUCTURE OF THE HUMAN VILLIN C-TERMINAL HEADPIECE SUBDOMAIN

Template:ABSTRACT PUBMED 15096633

About this Structure

1UNC is a Single protein structure. Full experimental information is available from OCA.

Reference

Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements., Vermeulen W, Vanhaesebrouck P, Van Troys M, Verschueren M, Fant F, Goethals M, Ampe C, Martins JC, Borremans FA, Protein Sci. 2004 May;13(5):1276-87. PMID:15096633

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