2rbi

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{{STRUCTURE_2rbi| PDB=2rbi | SCENE= }}
{{STRUCTURE_2rbi| PDB=2rbi | SCENE= }}
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'''STRUCTURE OF BINASE MUTANT HIS 101 ASN'''
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===STRUCTURE OF BINASE MUTANT HIS 101 ASN===
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==Overview==
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Members of the microbial guanyl-specific ribonuclease family catalyse the endonucleolytic cleavage of single-stranded RNA in a two-step reaction involving transesterification to form a 2',3'-cyclic phosphate and its subsequent hydrolysis to yield the respective 3'-phosphate. The extracellular ribonuclease from Bacillus intermedius (binase, RNase Bi) shares a common mechanism for RNA hydrolysis with mammalian RNases. Two catalytic residues in the active site of binase, Glu72 and His101, are thought to be involved in general acid-general base catalysis of RNA cleavage. Using site-directed mutagenesis, binase mutants were produced containing amino acid substitutions H101N and H101T and their catalytic properties towards RNA, poly(I), poly(A), GpC and guanosine 2',3'-cyclic phosphate (cGMP) substrates were studied. The engineered mutant proteins are active in the transesterification step which produces the 2',3'-cyclic phosphate species but they have lost the ability to catalyse hydrolysis of the cyclic phosphate to give the 3' monophosphate product.
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(as it appears on PubMed at http://www.pubmed.gov), where 9153077 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9153077}}
==About this Structure==
==About this Structure==
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[[Category: Microbial extracellular ribonuclease]]
[[Category: Microbial extracellular ribonuclease]]
[[Category: Phosphodiester transferase]]
[[Category: Phosphodiester transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:36:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:26:39 2008''

Revision as of 12:26, 29 July 2008

Image:2rbi.png

Template:STRUCTURE 2rbi

STRUCTURE OF BINASE MUTANT HIS 101 ASN

Template:ABSTRACT PUBMED 9153077

About this Structure

2RBI is a Single protein structure of sequence from Bacillus intermedius. Full crystallographic information is available from OCA.

Reference

RNA cleavage without hydrolysis. Splitting the catalytic activities of binase with Asn101 and Thr101 mutations., Okorokov AL, Panov KI, Offen WA, Mukhortov VG, Antson AA, Karpeisky MYa, Wilkinson AJ, Dodson GG, Protein Eng. 1997 Mar;10(3):273-8. PMID:9153077

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