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| - | [[Image:2om3.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2om3| PDB=2om3 | SCENE= }} | | {{STRUCTURE_2om3| PDB=2om3 | SCENE= }} |
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| - | '''High-resolution cryo-EM structure of Tobacco Mosaic Virus'''
| + | ===High-resolution cryo-EM structure of Tobacco Mosaic Virus=== |
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| - | ==Overview==
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| - | The treatment of helical objects as a string of single particles has become an established technique to resolve their three-dimensional (3D) structure using electron cryo-microscopy. It can be applied to a wide range of helical particles such as viruses, microtubules and helical filaments. We have made improvements to this approach using Tobacco Mosaic Virus (TMV) as a test specimen and obtained a map from 210,000 asymmetric units at a resolution better than 5 A. This was made possible by performing a full correction of the contrast transfer function of the microscope. Alignment of helical segments was helped by constraints derived from the helical symmetry of the virus. Furthermore, symmetrization was implemented by multiple inclusions of symmetry-related views in the 3D reconstruction. We used the density map to build an atomic model of TMV. The model was refined using a real-space refinement strategy that accommodates multiple conformers. The atomic model shows significant deviations from the deposited model for the helical form of TMV at the lower-radius region (residues 88 to 109). This region appears more ordered with well-defined secondary structure, compared with the earlier helical structure. The RNA phosphate backbone is sandwiched between two arginine side-chains, stabilizing the interaction between RNA and coat protein. A cluster of two or three carboxylates is buried in a hydrophobic environment isolating it from neighboring subunits. These carboxylates may represent the so-called Caspar carboxylates that form a metastable switch for viral disassembly. Overall, the observed differences suggest that the new model represents a different, more stable state of the virus, compared with the earlier published model. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17585939}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17585939 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17585939}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Helical virus]] | | [[Category: Helical virus]] |
| | [[Category: Protein-rna complex]] | | [[Category: Protein-rna complex]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:11:53 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:27:52 2008'' |
Revision as of 12:27, 29 July 2008
Template:STRUCTURE 2om3
High-resolution cryo-EM structure of Tobacco Mosaic Virus
Template:ABSTRACT PUBMED 17585939
About this Structure
2OM3 is a Single protein structure of sequence from Tobacco mosaic virus. Full crystallographic information is available from OCA.
Reference
High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virus., Sachse C, Chen JZ, Coureux PD, Stroupe ME, Fandrich M, Grigorieff N, J Mol Biol. 2007 Aug 17;371(3):812-35. Epub 2007 Jun 4. PMID:17585939
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