2r66

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{{STRUCTURE_2r66| PDB=2r66 | SCENE= }}
{{STRUCTURE_2r66| PDB=2r66 | SCENE= }}
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'''Complex Structure of Sucrose Phosphate Synthase (SPS)-F6P of Halothermothrix orenii'''
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===Complex Structure of Sucrose Phosphate Synthase (SPS)-F6P of Halothermothrix orenii===
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==Overview==
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Sucrose phosphate synthase (SPS) catalyzes the transfer of a glycosyl group from an activated donor sugar, such as uridine diphosphate glucose (UDP-Glc), to a saccharide acceptor d-fructose 6-phosphate (F6P), resulting in the formation of UDP and d-sucrose-6'-phosphate (S6P). This is a central regulatory process in the production of sucrose in plants, cyanobacteria, and proteobacteria. Here, we report the crystal structure of SPS from the nonphotosynthetic bacterium Halothermothrix orenii and its complexes with the substrate F6P and the product S6P. SPS has two distinct Rossmann-fold domains with a large substrate binding cleft at the interdomain interface. Structures of two complexes show that both the substrate F6P and the product S6P bind to the A-domain of SPS. Based on comparative analysis of the SPS structure with other related enzymes, the donor substrate, nucleotide diphosphate glucose, binds to the B-domain of SPS. Furthermore, we propose a mechanism of catalysis by H. orenii SPS. Our findings indicate that SPS from H. orenii may represent a valid model for the catalytic domain of plant SPSs and thus may provide useful insight into the reaction mechanism of the plant enzyme.
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{{ABSTRACT_PUBMED_18424616}}
==About this Structure==
==About this Structure==
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[[Category: Rossmann-fold]]
[[Category: Rossmann-fold]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:44:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:36:00 2008''

Revision as of 12:36, 29 July 2008

Image:2r66.png

Template:STRUCTURE 2r66

Complex Structure of Sucrose Phosphate Synthase (SPS)-F6P of Halothermothrix orenii

Template:ABSTRACT PUBMED 18424616

About this Structure

2R66 is a Single protein structure of sequence from Halothermothrix orenii. Full crystallographic information is available from OCA.

Reference

The Structure of Sucrose Phosphate Synthase from Halothermothrix orenii Reveals Its Mechanism of Action and Binding Mode., Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J, Plant Cell. 2008 Apr;20(4):1059-72. Epub 2008 Apr 18. PMID:18424616

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