2p81

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{{STRUCTURE_2p81| PDB=2p81 | SCENE= }}
{{STRUCTURE_2p81| PDB=2p81 | SCENE= }}
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'''Engrailed homeodomain helix-turn-helix motif'''
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===Engrailed homeodomain helix-turn-helix motif===
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==Overview==
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Helices 2 and 3 of Engrailed homeodomain (EnHD) form a helix-turn-helix (HTH) motif. This common motif is believed not to fold independently, which is the characteristic feature of a motif rather than a domain. But we found that the EnHD HTH motif is monomeric and folded in solution, having essentially the same structure as in full-length protein. It had a sigmoidal thermal denaturation transition. Both native backbone and local tertiary interactions were formed concurrently at 4 x 10(5) s(-1) at 25 degrees C, monitored by IR and fluorescence T-jump kinetics, respectively, the same rate constant as for the fast phase in the folding of EnHD. The HTH motif, thus, is an ultrafast-folding, natural protein domain. Its independent stability and appropriate folding kinetics account for the stepwise folding of EnHD, satisfy fully the criteria for an on-pathway intermediate, and explain the changes in mechanism of folding across the homeodomain family. Experiments on mutated and engineered fragments of the parent protein with different probes allowed the assignment of the observed kinetic phases to specific events to show that EnHD is not an example of one-state downhill folding.
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(as it appears on PubMed at http://www.pubmed.gov), where 17517666 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17517666}}
==About this Structure==
==About this Structure==
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2P81 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P81 OCA].
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2P81 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P81 OCA].
==Reference==
==Reference==
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[[Category: Native]]
[[Category: Native]]
[[Category: Protein folding]]
[[Category: Protein folding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:34:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:43:33 2008''

Revision as of 12:43, 29 July 2008

Image:2p81.png

Template:STRUCTURE 2p81

Engrailed homeodomain helix-turn-helix motif

Template:ABSTRACT PUBMED 17517666

About this Structure

2P81 is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

The helix-turn-helix motif as an ultrafast independently folding domain: the pathway of folding of Engrailed homeodomain., Religa TL, Johnson CM, Vu DM, Brewer SH, Dyer RB, Fersht AR, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9272-7. Epub 2007 May 18. PMID:17517666

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