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1up7

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{{STRUCTURE_1up7| PDB=1up7 | SCENE= }}
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'''STRUCTURE OF THE 6-PHOSPHO-BETA GLUCOSIDASE FROM THERMOTOGA MARITIMA AT 2.4 ANSTROM RESOLUTION IN THE TETRAGONAL FORM WITH NAD AND GLUCOSE-6-PHOSPHATE'''
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===STRUCTURE OF THE 6-PHOSPHO-BETA GLUCOSIDASE FROM THERMOTOGA MARITIMA AT 2.4 ANSTROM RESOLUTION IN THE TETRAGONAL FORM WITH NAD AND GLUCOSE-6-PHOSPHATE===
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==Overview==
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The import of disaccharides by many bacteria is achieved through their simultaneous translocation and phosphorylation by the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). The imported phospho-disaccharides are, in some cases, subsequently hydrolyzed by members of the unusual glycoside hydrolase family GH4. The GH4 enzymes, occasionally found also in bacteria such as Thermotoga maritima that do not utilise a PEP-PTS system, require both NAD(+) and Mn(2+) for catalysis. A further curiosity of this family is that closely related enzymes may show specificity for either alpha-d- or beta-d-glycosides. Here, we present, for the first time, the three-dimensional structure (using single-wavelength anomalous dispersion methods, harnessing extensive non-crystallographic symmetry) of the 6-phospho-beta-glycosidase, BglT, from T.maritima in native and complexed (NAD(+) and Glc6P) forms. Comparison of the active-center structure with that of the 6-phospho-alpha-glucosidase GlvA from Bacillus subtilis reveals a striking degree of structural similarity that, in light of previous kinetic isotope effect data, allows the postulation of a common reaction mechanism for both alpha and beta-glycosidases. Given that the "chemistry" occurs primarily on the glycone sugar and features no nucleophilic attack on the intact disaccharide substrate, modulation of anomeric specificity for alpha and beta-linkages is accommodated through comparatively minor structural changes.
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{{ABSTRACT_PUBMED_15670594}}
==About this Structure==
==About this Structure==
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[[Category: Family4 hydrolase]]
[[Category: Family4 hydrolase]]
[[Category: Nad dependent]]
[[Category: Nad dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:31:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:51:41 2008''

Revision as of 12:51, 29 July 2008

Image:1up7.png

Template:STRUCTURE 1up7

STRUCTURE OF THE 6-PHOSPHO-BETA GLUCOSIDASE FROM THERMOTOGA MARITIMA AT 2.4 ANSTROM RESOLUTION IN THE TETRAGONAL FORM WITH NAD AND GLUCOSE-6-PHOSPHATE

Template:ABSTRACT PUBMED 15670594

About this Structure

1UP7 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

NAD+ and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides., Varrot A, Yip VL, Li Y, Rajan SS, Yang X, Anderson WF, Thompson J, Withers SG, Davies GJ, J Mol Biol. 2005 Feb 18;346(2):423-35. Epub 2005 Jan 7. PMID:15670594

Page seeded by OCA on Tue Jul 29 15:51:41 2008

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