2z1j

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{{STRUCTURE_2z1j| PDB=2z1j | SCENE= }}
{{STRUCTURE_2z1j| PDB=2z1j | SCENE= }}
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'''Crystal structure of E.coli RNase HI surface charged mutant(Q4R/T40E/Q72H/Q76K/Q80E/T92K/Q105K/Q113R/Q115K/N143K/T145K)'''
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===Crystal structure of E.coli RNase HI surface charged mutant(Q4R/T40E/Q72H/Q76K/Q80E/T92K/Q105K/Q113R/Q115K/N143K/T145K)===
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==Overview==
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Using the information from the genome projects, recent comparative studies of thermostable proteins have revealed a certain trend of amino acid composition in which polar residues are scarce and charged residues are rich on the protein surface. To clarify experimentally the effect of the amino acid composition of surface residues on the thermostability of Escherichia coli Ribonuclease HI (RNase HI), we constructed six variants in which five to eleven polar residues were replaced by charged residues (5C, 7Ca, 7Cb, 9Ca, 9Cb and 11C). The thermal denaturation experiments indicated that all of the variant proteins are 3.2-10.1 degrees C in Tm less stable than the wild proteins. The crystal structures of resultant protein variants 7Ca, 7Cb, 9Ca and 11C closely resemble that of E. coli RNase HI in their global fold, and several different hydrogen bonding and ion-pair interactions are formed by the mutations. Comparison of the crystal structures of these variant proteins with that of E. coli RNase HI reveals that thermal destabilization is apparently related to electrostatic repulsion of the charged residues with neighbours. This result suggests that charged residues of natural thermostable proteins are strictly posted on the surface with optimal interactions and without repulsive interactions.
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{{ABSTRACT_PUBMED_17761696}}
==About this Structure==
==About this Structure==
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[[Category: Surface-charge residue]]
[[Category: Surface-charge residue]]
[[Category: Thermostability]]
[[Category: Thermostability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:55:51 2008''

Revision as of 12:55, 29 July 2008

Image:2z1j.png

Template:STRUCTURE 2z1j

Crystal structure of E.coli RNase HI surface charged mutant(Q4R/T40E/Q72H/Q76K/Q80E/T92K/Q105K/Q113R/Q115K/N143K/T145K)

Template:ABSTRACT PUBMED 17761696

About this Structure

2Z1J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Protein thermostabilization requires a fine-tuned placement of surface-charged residues., You DJ, Fukuchi S, Nishikawa K, Koga Y, Takano K, Kanaya S, J Biochem. 2007 Oct;142(4):507-16. Epub 2007 Aug 30. PMID:17761696

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