1zwu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1zwu.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1zwu.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1zwu| PDB=1zwu | SCENE= }}
{{STRUCTURE_1zwu| PDB=1zwu | SCENE= }}
-
'''30 NMR structures of AcAMP2-like peptide with non natural beta-(2-naphthyl)-alanine residue.'''
+
===30 NMR structures of AcAMP2-like peptide with non natural beta-(2-naphthyl)-alanine residue.===
-
==Overview==
+
<!--
-
The specific interaction of a variety of modified hevein domains to chitooligosaccharides has been studied by NMR spectroscopy in order to assess the importance of aromatic-carbohydrate interactions for the molecular recognition of neutral sugars. These mutant AcAMP2-like peptides, which have 4-fluoro-phenylalanine, tryptophan, or 2-naphthylalanine at the key interacting positions, have been prepared by solid-phase synthesis. Their three-dimensional structures, when bound to the chitin-derived trisaccharide, have been deduced by NMR spectroscopy. By using DYANA and restrained molecular dynamics simulations with the AMBER 5.0 force field, the three-dimensional structures of the protein-sugar complexes have been obtained. The thermodynamic analysis of the interactions that occur upon complex formation have also been carried out. Regarding binding affinity, the obtained data have permitted the deduction that the larger the aromatic group, the higher the association constant and the binding enthalpy. In all cases, entropy opposes binding. In contrast, deactivation of the aromatic rings by attaching fluorine atoms decreases the binding affinity, with a concomitant decrease in enthalpy. The role of the chemical nature of the aromatic ring for establishing sugar contacts has been thus evaluated.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16220560}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16220560 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16220560}}
==About this Structure==
==About this Structure==
-
1ZWU is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWU OCA].
+
1ZWU is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWU OCA].
==Reference==
==Reference==
On the importance of carbohydrate-aromatic interactions for the molecular recognition of oligosaccharides by proteins: NMR studies of the structure and binding affinity of AcAMP2-like peptides with non-natural naphthyl and fluoroaromatic residues., Chavez MI, Andreu C, Vidal P, Aboitiz N, Freire F, Groves P, Asensio JL, Asensio G, Muraki M, Canada FJ, Jimenez-Barbero J, Chemistry. 2005 Nov 18;11(23):7060-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16220560 16220560]
On the importance of carbohydrate-aromatic interactions for the molecular recognition of oligosaccharides by proteins: NMR studies of the structure and binding affinity of AcAMP2-like peptides with non-natural naphthyl and fluoroaromatic residues., Chavez MI, Andreu C, Vidal P, Aboitiz N, Freire F, Groves P, Asensio JL, Asensio G, Muraki M, Canada FJ, Jimenez-Barbero J, Chemistry. 2005 Nov 18;11(23):7060-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16220560 16220560]
 +
 +
H NMR study of the solution structure of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus., Martins JC, Maes D, Loris R, Pepermans HA, Wyns L, Willem R, Verheyden P, J Mol Biol. 1996 May 3;258(2):322-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8627629 8627629]
 +
 +
The importance of CH/pi interactions to the function of carbohydrate binding proteins., Muraki M, Protein Pept Lett. 2002 Jun;9(3):195-209. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12144516 12144516]
 +
 +
NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides., Aboitiz N, Vila-Perello M, Groves P, Asensio JL, Andreu D, Canada FJ, Jimenez-Barbero J, Chembiochem. 2004 Sep 6;5(9):1245-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15368576 15368576]
 +
 +
NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex between pseudohevein and N,N',N"-triacetylchitotriose., Asensio JL, Siebert HC, von Der Lieth CW, Laynez J, Bruix M, Soedjanaamadja UM, Beintema JJ, Canada FJ, Gabius HJ, Jimenez-Barbero J, Proteins. 2000 Aug 1;40(2):218-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10842338 10842338]
 +
 +
Structural basis for chitin recognition by defense proteins: GlcNAc residues are bound in a multivalent fashion by extended binding sites in hevein domains., Asensio JL, Canada FJ, Siebert HC, Laynez J, Poveda A, Nieto PM, Soedjanaamadja UM, Gabius HJ, Jimenez-Barbero J, Chem Biol. 2000 Jul;7(7):529-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10903932 10903932]
 +
 +
Chemically prepared hevein domains: effect of C-terminal truncation and the mutagenesis of aromatic residues on the affinity for chitin., Muraki M, Morii H, Harata K, Protein Eng. 2000 Jun;13(6):385-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10877847 10877847]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Aboitiz, N.]]
[[Category: Aboitiz, N.]]
Line 34: Line 50:
[[Category: Alpha-helix]]
[[Category: Alpha-helix]]
[[Category: Anti-parallel beta-sheet.]]
[[Category: Anti-parallel beta-sheet.]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:10:34 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:56:25 2008''

Revision as of 12:56, 29 July 2008

Image:1zwu.png

Template:STRUCTURE 1zwu

30 NMR structures of AcAMP2-like peptide with non natural beta-(2-naphthyl)-alanine residue.

Template:ABSTRACT PUBMED 16220560

About this Structure

1ZWU is a Single protein structure. Full experimental information is available from OCA.

Reference

On the importance of carbohydrate-aromatic interactions for the molecular recognition of oligosaccharides by proteins: NMR studies of the structure and binding affinity of AcAMP2-like peptides with non-natural naphthyl and fluoroaromatic residues., Chavez MI, Andreu C, Vidal P, Aboitiz N, Freire F, Groves P, Asensio JL, Asensio G, Muraki M, Canada FJ, Jimenez-Barbero J, Chemistry. 2005 Nov 18;11(23):7060-74. PMID:16220560

H NMR study of the solution structure of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus., Martins JC, Maes D, Loris R, Pepermans HA, Wyns L, Willem R, Verheyden P, J Mol Biol. 1996 May 3;258(2):322-33. PMID:8627629

The importance of CH/pi interactions to the function of carbohydrate binding proteins., Muraki M, Protein Pept Lett. 2002 Jun;9(3):195-209. PMID:12144516

NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides., Aboitiz N, Vila-Perello M, Groves P, Asensio JL, Andreu D, Canada FJ, Jimenez-Barbero J, Chembiochem. 2004 Sep 6;5(9):1245-55. PMID:15368576

NMR investigations of protein-carbohydrate interactions: studies on the relevance of Trp/Tyr variations in lectin binding sites as deduced from titration microcalorimetry and NMR studies on hevein domains. Determination of the NMR structure of the complex between pseudohevein and N,N',N"-triacetylchitotriose., Asensio JL, Siebert HC, von Der Lieth CW, Laynez J, Bruix M, Soedjanaamadja UM, Beintema JJ, Canada FJ, Gabius HJ, Jimenez-Barbero J, Proteins. 2000 Aug 1;40(2):218-36. PMID:10842338

Structural basis for chitin recognition by defense proteins: GlcNAc residues are bound in a multivalent fashion by extended binding sites in hevein domains., Asensio JL, Canada FJ, Siebert HC, Laynez J, Poveda A, Nieto PM, Soedjanaamadja UM, Gabius HJ, Jimenez-Barbero J, Chem Biol. 2000 Jul;7(7):529-43. PMID:10903932

Chemically prepared hevein domains: effect of C-terminal truncation and the mutagenesis of aromatic residues on the affinity for chitin., Muraki M, Morii H, Harata K, Protein Eng. 2000 Jun;13(6):385-9. PMID:10877847

Page seeded by OCA on Tue Jul 29 15:56:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zwu"
Personal tools