2d7s

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{{STRUCTURE_2d7s| PDB=2d7s | SCENE= }}
{{STRUCTURE_2d7s| PDB=2d7s | SCENE= }}
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'''Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with VPg protein'''
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===Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with VPg protein===
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==Overview==
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Picornavirus RNA replication is initiated by the covalent attachment of a UMP molecule to the hydroxyl group of a tyrosine in the terminal protein VPg. This reaction is carried out by the viral RNA-dependent RNA polymerase (3D). Here, we report the X-ray structure of two complexes between foot-and-mouth disease virus 3D, VPg1, the substrate UTP and divalent cations, in the absence and in the presence of an oligoadenylate of 10 residues. In both complexes, VPg fits the RNA binding cleft of the polymerase and projects the key residue Tyr3 into the active site of 3D. This is achieved by multiple interactions with residues of motif F and helix alpha8 of the fingers domain and helix alpha13 of the thumb domain of the polymerase. The complex obtained in the presence of the oligoadenylate showed the product of the VPg uridylylation (VPg-UMP). Two metal ions and the catalytic aspartic acids of the polymerase active site, together with the basic residues of motif F, have been identified as participating in the priming reaction.
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{{ABSTRACT_PUBMED_16456546}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16456546 16456546]
The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16456546 16456546]
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Structure of foot-and-mouth disease virus RNA-dependent RNA polymerase and its complex with a template-primer RNA., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, J Biol Chem. 2004 Nov 5;279(45):47212-21. Epub 2004 Aug 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15294895 15294895]
[[Category: Foot and mouth disease virus]]
[[Category: Foot and mouth disease virus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Rna-dependent rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Vpg]]
[[Category: Vpg]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:58:40 2008''

Revision as of 12:58, 29 July 2008

Image:2d7s.png

Template:STRUCTURE 2d7s

Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with VPg protein

Template:ABSTRACT PUBMED 16456546

About this Structure

2D7S is a Protein complex structure of sequences from Foot and mouth disease virus. Full crystallographic information is available from OCA.

Reference

The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:16456546

Structure of foot-and-mouth disease virus RNA-dependent RNA polymerase and its complex with a template-primer RNA., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, J Biol Chem. 2004 Nov 5;279(45):47212-21. Epub 2004 Aug 3. PMID:15294895

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