2gmr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2gmr.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2gmr.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2gmr| PDB=2gmr | SCENE= }}
{{STRUCTURE_2gmr| PDB=2gmr | SCENE= }}
-
'''Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn'''
+
===Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn===
-
==Overview==
+
<!--
-
The reaction center (RC) of Rhodobacter sphaeroides uses light energy to reduce and protonate a quinone molecule, QB (the secondary quinone electron acceptor), to form quinol, QBH2. Asp210 in the L-subunit has been shown to be a catalytic residue in this process. Mutation of Asp210 to Asn leads to a deceleration of reoxidation of QA- in the QA-QB --&gt; QAQB- transition. Here we determined the structure of the Asp210 to Asn mutant to 2.5 A and show that there are no major structural differences as compared to the wild-type protein. We found QB in the distal position and a chain of water molecules between Asn210 and QB. Using time-resolved Fourier transform infrared (trFTIR) spectroscopy, we characterized the molecular reaction mechanism of this mutant. We found that QB- formation precedes QA- oxidation even more pronounced than in the wild-type reaction center. Continuum absorbance changes indicate deprotonation of a protonated water cluster, most likely of the water chain between Asn210 and QB. A detailed analysis of wild-type structures revealed a highly conserved water chain between Asp210 or Glu210 and QB in Rb. sphaeroides and Rhodopseudomonas viridis, respectively.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17105193}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17105193 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17105193}}
==About this Structure==
==About this Structure==
Line 33: Line 37:
[[Category: Proton transfer]]
[[Category: Proton transfer]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:17:00 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:59:20 2008''

Revision as of 12:59, 29 July 2008

Image:2gmr.png

Template:STRUCTURE 2gmr

Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn

Template:ABSTRACT PUBMED 17105193

About this Structure

2GMR is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules., Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K, Biochemistry. 2006 Nov 21;45(46):13741-9. PMID:17105193

Page seeded by OCA on Tue Jul 29 15:59:20 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gmr"
Personal tools