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2ggr

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[[Image:2ggr.gif|left|200px]]
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{{STRUCTURE_2ggr| PDB=2ggr | SCENE= }}
{{STRUCTURE_2ggr| PDB=2ggr | SCENE= }}
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'''Solution structure of the C-terminal SH3 domain of c-CrkII'''
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===Solution structure of the C-terminal SH3 domain of c-CrkII===
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==Overview==
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Crk-II is a signaling adaptor protein that is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an Src homology 2 domain (SH2) followed by two Src homology 3 (SH3) domains. The structures and ligand-binding properties of the SH2 and the middle SH3 domains are well-characterized. Several studies suggest that the C-terminal SH3 domain plays an important regulatory role in the protein; however, no structural information is available on this domain, and relatively little is known about its binding partners. In the current work, we have solved the solution NMR structure of the C-terminal SH3 domain. The domain adopts the standard SH3 fold comprising a five-stranded beta barrel. In agreement with alignment and modeling studies, the structure indicates that the canonical-binding surface of the SH3 domain is unusually polar and suggests that this domain may not bind typical PXXP ligands or that it may bind them with reduced affinity. Thermodynamic and kinetic studies show that the domain folds in a reversible two-state manner and that the stability of the fold is similar to that observed for other SH3 domains. These studies offer some insight into the likely structural and thermodynamic consequences of point mutations in the cSH3 domain that are known to deregulate Crk-II function. Our results set the stage for a better understanding the role of the cSH3 domain in the context of the full-length protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 16846230 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16846230}}
==About this Structure==
==About this Structure==
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2GGR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GGR OCA].
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2GGR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GGR OCA].
==Reference==
==Reference==
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[[Category: Sh3 domain]]
[[Category: Sh3 domain]]
[[Category: Solution structure]]
[[Category: Solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:05:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:01:16 2008''

Revision as of 13:01, 29 July 2008

Image:2ggr.png

Template:STRUCTURE 2ggr

Solution structure of the C-terminal SH3 domain of c-CrkII

Template:ABSTRACT PUBMED 16846230

About this Structure

2GGR is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Solution structure and folding characteristics of the C-terminal SH3 domain of c-Crk-II., Muralidharan V, Dutta K, Cho J, Vila-Perello M, Raleigh DP, Cowburn D, Muir TW, Biochemistry. 2006 Jul 25;45(29):8874-84. PMID:16846230

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