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| - | [[Image:1v8p.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1v8p| PDB=1v8p | SCENE= }} | | {{STRUCTURE_1v8p| PDB=1v8p | SCENE= }} |
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| - | '''Crystal structure of PAE2754 from Pyrobaculum aerophilum'''
| + | ===Crystal structure of PAE2754 from Pyrobaculum aerophilum=== |
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| - | ==Overview==
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| - | Genome sequencing projects have focused attention on the problem of discovering the functions of protein domains that are widely distributed throughout living species but which are, as yet, largely uncharacterized. One such example is the PIN domain, found in eukaryotes, bacteria, and Archaea, and with suggested roles in signaling, RNase editing, and/or nucleotide binding. The first reported crystal structure of a PIN domain (open reading frame PAE2754, derived from the crenarchaeon, Pyrobaculum aerophilum) has been determined to 2.5 A resolution and is presented here. Mapping conserved residues from a multiple sequence alignment onto the structure identifies a putative active site. The discovery of distant structural homology with several exonucleases, including T4 phage RNase H and flap endonuclease (FEN1), further suggests a likely function for PIN domains as Mg2+-dependent exonucleases, a hypothesis that we have confirmed in vitro. The tetrameric structure of PAE2754, with the active sites inside a tunnel, suggests a mechanism for selective cleavage of single-stranded overhangs or flap structures. These results indicate likely DNA or RNA editing roles for prokaryotic PIN domains, which are strikingly numerous in thermophiles, and in organisms such as Mycobacterium tuberculosis. They also support previous hypotheses that eukaryotic PIN domains participate in RNAi and nonsense-mediated RNA degradation.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_14734548}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 14734548 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_14734548}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| | [[Category: Tetramer]] | | [[Category: Tetramer]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:13:42 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:01:22 2008'' |
Revision as of 13:01, 29 July 2008
Template:STRUCTURE 1v8p
Crystal structure of PAE2754 from Pyrobaculum aerophilum
Template:ABSTRACT PUBMED 14734548
About this Structure
1V8P is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.
Reference
Distant structural homology leads to the functional characterization of an archaeal PIN domain as an exonuclease., Arcus VL, Backbro K, Roos A, Daniel EL, Baker EN, J Biol Chem. 2004 Apr 16;279(16):16471-8. Epub 2004 Jan 20. PMID:14734548
Page seeded by OCA on Tue Jul 29 16:01:22 2008
