2af2

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[[Image:2af2.gif|left|200px]]
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{{STRUCTURE_2af2| PDB=2af2 | SCENE= }}
{{STRUCTURE_2af2| PDB=2af2 | SCENE= }}
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'''Solution structure of disulfide reduced and copper depleted Human Superoxide Dismutase'''
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===Solution structure of disulfide reduced and copper depleted Human Superoxide Dismutase===
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==Overview==
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SOD1 has to undergo several post-translational modifications before reaching its mature form. The protein requires insertion of zinc and copper atoms, followed by the formation of a conserved S-S bond between Cys-57 and Cys-146 (human numbering), which makes the protein fully active. In this report an NMR structural investigation of the reduced SH-SH form of thermostable E,Zn-as-SOD1 (E is empty; as is C6A, C111S) is reported, characterizing the protein just before the last step leading to the mature form. The structure is compared with that of the oxidized S-S form as well as with that of the yeast SOD1 complexed with its copper chaperone, CCS. Local conformational rearrangements upon disulfide bridge reduction are localized in the region near Cys-57 that is completely exposed to the solvent in the present structure, at variance with the oxidized forms. There is a local disorder around Cys-57 that may serve for protein-protein recognition and may possibly be involved in intermolecular S-S bonds in familial amyotrophic lateral sclerosis-related SOD1 mutants. The structure allows us to further discuss the copper loading mechanism in SOD1.
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The line below this paragraph, {{ABSTRACT_PUBMED_16291742}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16291742 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16291742}}
==Disease==
==Disease==
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==About this Structure==
==About this Structure==
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2AF2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF2 OCA].
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2AF2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AF2 OCA].
==Reference==
==Reference==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Structural proteomics in europe]]
[[Category: Structural proteomics in europe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:17:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:02:11 2008''

Revision as of 13:02, 29 July 2008

Image:2af2.png

Template:STRUCTURE 2af2

Contents

Solution structure of disulfide reduced and copper depleted Human Superoxide Dismutase

Template:ABSTRACT PUBMED 16291742

Disease

Known disease associated with this structure: Amyotrophic lateral sclerosis, due to SOD1 deficiency OMIM:[147450]

About this Structure

2AF2 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Human SOD1 before harboring the catalytic metal: solution structure of copper-depleted, disulfide-reduced form., Banci L, Bertini I, Cantini F, D'Amelio N, Gaggelli E, J Biol Chem. 2006 Jan 27;281(4):2333-7. Epub 2005 Nov 14. PMID:16291742

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