1qma
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(New page: 200px<br /><applet load="1qma" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qma, resolution 2.5Å" /> '''NUCLEAR TRANSPORT FAC...)
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Revision as of 22:41, 20 November 2007
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NUCLEAR TRANSPORT FACTOR 2 (NTF2) W7A MUTANT
Overview
Nuclear transport factor 2 (NTF2) is a small, homodimeric protein that, binds to both RanGDP and xFxFG repeat-containing nucleoporins, such as, yeast Nsp1p and vertebrate p62. NTF2 is required for efficient nuclear, protein import and has been shown to mediate the nuclear import of RanGDP., We have used the crystal structures of rat NTF2 and its complex with, RanGDP to design a mutant, W7A-NTF2, in which the affinity for, xFxFG-repeat nucleoporins is reduced while wild-type binding to RanGDP is, retained. The 2.5 A resolution crystal structure of W7A-NTF2 is virtually, superimposable upon the wild-type protein structure, indicating that the, mutation had not introduced a more general conformational change., Therefore, our data suggest that the exposed side-chain of residue 7 is, crucial to the interaction between NTF2 and xFxFG repeat-containing, nucleoporins. Consistent with its reduced affinity for xFxFG nucleoporins, fluorescently labelled W7A-NTF2 binds less strongly to the nuclear, envelope of permeabilized cultured cells than wild-type NTF2 and, when, microinjected into Xenopus oocytes, colloidal gold coated with W7A-NTF2, binds less strongly to the central channel of nuclear pore complexes than, wild-type NTF2-coated gold. Significantly, W7A-NTF2 only weakly stimulated, the nuclear import of fluorescein-labelled RanGDP, providing direct, evidence that an interaction between NTF2 and xFxFG repeat-containing, nucleoporins is required to mediate the nuclear import of RanGDP.
About this Structure
1QMA is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP., Bayliss R, Ribbeck K, Akin D, Kent HM, Feldherr CM, Gorlich D, Stewart M, J Mol Biol. 1999 Oct 29;293(3):579-93. PMID:10543952
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