1plw

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[[Image:1plw.gif|left|200px]]
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{{Seed}}
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[[Image:1plw.png|left|200px]]
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{{STRUCTURE_1plw| PDB=1plw | SCENE= }}
{{STRUCTURE_1plw| PDB=1plw | SCENE= }}
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'''NMR structure of Methionine-Enkephalin in fast tumbling DMPC/DHPC bicelles'''
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===NMR structure of Methionine-Enkephalin in fast tumbling DMPC/DHPC bicelles===
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==Overview==
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Enkephalins are pentapeptides found in the central nervous system. It is believed that these neuropeptides interact with the nerve cell membrane to adopt a conformation suitable for their binding to an opiate receptor. In this work, we have determined the three-dimensional structure of methionine-enkephalin (Menk) in fast-tumbling bicelles using multidimensional (1)H NMR. Bicelles were selected as model membranes because both their bilayer organization and composition resemble those of natural biomembranes. The effect of the membrane composition on the peptide conformation was explored using both zwitterionic (PC bicelles) and negatively charged bicelles (Bic/PG). Pulsed field gradient experiments allowed the determination of the proportion of Menk bound to the model membranes. Approximately 60% of the water-soluble enkephalin was found to associate to the bicellar systems. Structure calculations from torsion angle and NOE-based distance constraints suggest the presence of both micro - and delta-selective conformers of Menk in each system and slightly different conformers in PC bicelles and Bic/PG. As opposed to previous studies of enkephalins in membrane mimetic systems, our results show that these opiate peptides could adopt several conformations in a membrane environment, which is consistent with the flexibility and poor selectivity of enkephalins.
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(as it appears on PubMed at http://www.pubmed.gov), where 14990485 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14990485}}
==About this Structure==
==About this Structure==
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1PLW is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLW OCA].
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1PLW is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLW OCA].
==Reference==
==Reference==
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[[Category: Separovic, F.]]
[[Category: Separovic, F.]]
[[Category: Peptide]]
[[Category: Peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:14:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:18:14 2008''

Revision as of 13:18, 29 July 2008

Image:1plw.png

Template:STRUCTURE 1plw

NMR structure of Methionine-Enkephalin in fast tumbling DMPC/DHPC bicelles

Template:ABSTRACT PUBMED 14990485

About this Structure

1PLW is a Single protein structure. Full experimental information is available from OCA.

Reference

A multidimensional 1H NMR investigation of the conformation of methionine-enkephalin in fast-tumbling bicelles., Marcotte I, Separovic F, Auger M, Gagne SM, Biophys J. 2004 Mar;86(3):1587-600. PMID:14990485

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