2exv

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{{STRUCTURE_2exv| PDB=2exv | SCENE= }}
{{STRUCTURE_2exv| PDB=2exv | SCENE= }}
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'''Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa'''
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===Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa===
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==Overview==
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Several investigators have highlighted a correlation between the basic features of the folding process of a protein and its topology, which dictates the folding pathway. Within this conceptual framework we proposed that different members of the cytochrome c (cyt c) family share the same folding mechanism, involving a consensus partially structured state. Pseudomonas aeruginosa cyt c(551) (Pa cyt c(551)) folds via an apparent two-state mechanism through a high energy intermediate. Here we present kinetic evidence demonstrating that it is possible to switch its folding mechanism from two to three state, stabilizing the high energy intermediate by rational mutagenesis. Characterization of the folding kinetics of one single-site mutant of the Pa cyt c(551) (Phe(7) to Ala) indeed reveals an additional refolding phase and a fast unfolding process which are explained by the accumulation of a partially folded species. Further kinetic analysis highlights the presence of two parallel processes both leading to the native state, suggesting that the above mentioned species is a non obligatory on-pathway intermediate. Determination of the crystallographic structure of F7A shows the presence of an extended internal cavity, which hosts three "bound" water molecules and a H-bond in the N-terminal helix, which is shorter than in the wild type protein. These two features allow us to propose a detailed structural interpretation for the stabilization of the native and especially the intermediate states induced by a single crucial mutation. These results show how protein engineering, x-ray crystallography and state-of-the-art kinetics concur to unveil a folding intermediate and the structural determinants of its stability.
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The line below this paragraph, {{ABSTRACT_PUBMED_16452476}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16452476 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16452476}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering., Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M, J Biol Chem. 2006 Apr 7;281(14):9331-6. Epub 2006 Feb 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16452476 16452476]
Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering., Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M, J Biol Chem. 2006 Apr 7;281(14):9331-6. Epub 2006 Feb 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16452476 16452476]
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Structure of cytochrome c551 from Pseudomonas aeruginosa refined at 1.6 A resolution and comparison of the two redox forms., Matsuura Y, Takano T, Dickerson RE, J Mol Biol. 1982 Apr 5;156(2):389-409. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6283101 6283101]
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Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart., Hasegawa J, Uchiyama S, Tanimoto Y, Mizutani M, Kobayashi Y, Sambongi Y, Igarashi Y, J Biol Chem. 2000 Dec 1;275(48):37824-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10918067 10918067]
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An obligatory intermediate in the folding pathway of cytochrome c552 from Hydrogenobacter thermophilus., Travaglini-Allocatelli C, Gianni S, Dubey VK, Borgia A, Di Matteo A, Bonivento D, Cutruzzola F, Bren KL, Brunori M, J Biol Chem. 2005 Jul 8;280(27):25729-34. Epub 2005 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15883159 15883159]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Cytochrome c]]
[[Category: Cytochrome c]]
[[Category: Heme c]]
[[Category: Heme c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:14:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:24:23 2008''

Revision as of 13:24, 29 July 2008

Image:2exv.png

Template:STRUCTURE 2exv

Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa

Template:ABSTRACT PUBMED 16452476

About this Structure

2EXV is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Unveiling a hidden folding intermediate in c-type cytochromes by protein engineering., Borgia A, Bonivento D, Travaglini-Allocatelli C, Di Matteo A, Brunori M, J Biol Chem. 2006 Apr 7;281(14):9331-6. Epub 2006 Feb 1. PMID:16452476

Structure of cytochrome c551 from Pseudomonas aeruginosa refined at 1.6 A resolution and comparison of the two redox forms., Matsuura Y, Takano T, Dickerson RE, J Mol Biol. 1982 Apr 5;156(2):389-409. PMID:6283101

Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart., Hasegawa J, Uchiyama S, Tanimoto Y, Mizutani M, Kobayashi Y, Sambongi Y, Igarashi Y, J Biol Chem. 2000 Dec 1;275(48):37824-8. PMID:10918067

An obligatory intermediate in the folding pathway of cytochrome c552 from Hydrogenobacter thermophilus., Travaglini-Allocatelli C, Gianni S, Dubey VK, Borgia A, Di Matteo A, Bonivento D, Cutruzzola F, Bren KL, Brunori M, J Biol Chem. 2005 Jul 8;280(27):25729-34. Epub 2005 May 9. PMID:15883159

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