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1jae

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{{STRUCTURE_1jae| PDB=1jae | SCENE= }}
{{STRUCTURE_1jae| PDB=1jae | SCENE= }}
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'''STRUCTURE OF TENEBRIO MOLITOR LARVAL ALPHA-AMYLASE'''
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===STRUCTURE OF TENEBRIO MOLITOR LARVAL ALPHA-AMYLASE===
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==Overview==
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The three-dimensional structure of the alpha-amylase from Tenebrio molitor larvae (TMA) has been determined by molecular replacement techniques using diffraction data of a crystal of space group P212121 (a=51.24 A; b=93.46 A; c=96.95 A). The structure has been refined to a crystallographic R-factor of 17.7% for 58,219 independent reflections in the 7.0 to 1.64 A resolution range, with root-mean-square deviations of 0.008 A for bond lengths and 1.482 degrees for bond angles. The final model comprises all 471 residues of TMA, 261 water molecules, one calcium cation and one chloride anion. The electron density confirms that the N-terminal glutamine residue has undergone a post-transitional modification resulting in a stable 5-oxo-proline residue. The X-ray structure of TMA provides the first three-dimensional model of an insect alpha-amylase.The monomeric enzyme exhibits an elongated shape approximately 75 Ax46 Ax40 A and consists of three distinct domains, in line with models for alpha-amylases from microbial, plant and mammalian origin. However, the structure of TMA reflects in the substrate and inhibitor binding region a remarkable difference from mammalian alpha-amylases: the lack of a highly flexible, glycine-rich loop, which has been proposed to be involved in a "trap-release" mechanism of substrate hydrolysis by mammalian alpha-amylases. The structural differences between alpha-amylases of various origins might explain the specificity of inhibitors directed exclusively against insect alpha-amylases.
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{{ABSTRACT_PUBMED_9600843}}
==About this Structure==
==About this Structure==
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[[Category: Glycosidase]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:58:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:28:37 2008''

Revision as of 13:28, 29 July 2008

Image:1jae.png

Template:STRUCTURE 1jae

STRUCTURE OF TENEBRIO MOLITOR LARVAL ALPHA-AMYLASE

Template:ABSTRACT PUBMED 9600843

About this Structure

1JAE is a Single protein structure of sequence from Tenebrio molitor. Full crystallographic information is available from OCA.

Reference

Crystal structure of yellow meal worm alpha-amylase at 1.64 A resolution., Strobl S, Maskos K, Betz M, Wiegand G, Huber R, Gomis-Ruth FX, Glockshuber R, J Mol Biol. 1998 May 8;278(3):617-28. PMID:9600843

Page seeded by OCA on Tue Jul 29 16:28:37 2008

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