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1xam

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{{STRUCTURE_1xam| PDB=1xam | SCENE= }}
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'''Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.'''
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===Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.===
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==Overview==
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We report here the crystal structure of the DNA hexamer duplex d(CGCGCA)*d(TGCGCG) at 1.71 A resolution. The crystals, in orthorhombic space group, were grown in the presence of cobalt hexammine, a known inducer of the left-handed Z form of DNA. The interaction of this ion with the DNA helix results in a change of the adenine base from the common amino tautomeric form to the imino tautomer. Consequently the A:T base pair is disrupted from the normal Watson-Crick base pairing to a 'wobble' like base pairing. This change is accommodated easily within the helix, and the helical parameters are those expected for Z-DNA. When the cobalt hexammine concentration is decreased slightly in the crystallization conditions, the duplex crystallizes in a different, hexagonal space group, with two hexamer duplexes in the asymmetric unit. One of these is situated on a crystallographic 6-fold screw axis, leading to disorder. The tautomeric shift is not observed in this space group. We show that the change in inter-helix interactions that lead to the two different space groups probably arise from the small decrease in ion concentration, and consequently disordered positions for the ion.
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(as it appears on PubMed at http://www.pubmed.gov), where 15534365 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15534365}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15534365 15534365]
Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15534365 15534365]
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Structure of d(TGCGCG).d(CGCGCA) in two crystal forms: effect of sequence and crystal packing in Z-DNA., Thiyagarajan S, Rajan SS, Gautham N, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1125-31. Epub 2005, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041078 16041078]
[[Category: Gautham, N.]]
[[Category: Gautham, N.]]
[[Category: Rajan, S S.]]
[[Category: Rajan, S S.]]
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Z-dna]]
[[Category: Z-dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:56:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:30:23 2008''

Revision as of 13:30, 29 July 2008

Image:1xam.png

Template:STRUCTURE 1xam

Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.

Template:ABSTRACT PUBMED 15534365

About this Structure

Full crystallographic information is available from OCA.

Reference

Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:15534365

Structure of d(TGCGCG).d(CGCGCA) in two crystal forms: effect of sequence and crystal packing in Z-DNA., Thiyagarajan S, Rajan SS, Gautham N, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1125-31. Epub 2005, Jul 20. PMID:16041078

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