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| | {{STRUCTURE_2ap5| PDB=2ap5 | SCENE= }} | | {{STRUCTURE_2ap5| PDB=2ap5 | SCENE= }} |
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| - | '''Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, Average Structure'''
| + | ===Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, Average Structure=== |
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| - | ==Overview==
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| - | The helical junction region of a -1 frameshift stimulating hairpin-type mRNA pseudoknot from sugarcane yellow leaf virus (ScYLV) is characterized by a novel C27.(G7-C14) loop 2-stem 1 minor groove base triple, which is stacked on a C8+.(G12-C28) loop 1-stem 2 major groove base triple. Substitution of C27 with adenosine reduces frameshifting efficiency to a level just twofold above the slip-site alone. Here, we show that the global structure of the C27A ScYLV RNA is nearly indistinguishable from the wild-type counterpart, despite the fact that the helical junction region is altered and incorporates the anticipated isostructural A27.(G7-C14) minor groove base triple. This interaction mediates a 2.3-A displacement of C8+ driven by an A27 N6-C8+ O2 hydrogen bond as part of an A(n-1).C+.G-Cn base quadruple. The helical junction regions of the C27A ScYLV and the beet western yellows virus (BWYV) pseudoknots are essentially superimposable, the latter of which contains an analogous A25.(G7-C14) minor groove base triple. These results reveal that the global ground-state structure is not strongly correlated with frameshift stimulation and point to a reduced thermodynamic stability and/or enhanced kinetic lability that derives from an altered helical junction architecture in the C27A ScYLV RNA as a significant determinant for setting frameshifting efficiencies in plant luteoviral mRNA pseudoknots. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17000902}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17000902 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17000902}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AP5 OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AP5 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Frameshifting]] | | [[Category: Frameshifting]] |
| | [[Category: Rna pseudoknot]] | | [[Category: Rna pseudoknot]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:18:27 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:32:58 2008'' |
Revision as of 13:33, 29 July 2008
Template:STRUCTURE 2ap5
Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, Average Structure
Template:ABSTRACT PUBMED 17000902
About this Structure
Full experimental information is available from OCA.
Reference
The global structures of a wild-type and poorly functional plant luteoviral mRNA pseudoknot are essentially identical., Cornish PV, Stammler SN, Giedroc DP, RNA. 2006 Nov;12(11):1959-69. Epub 2006 Sep 25. PMID:17000902
Page seeded by OCA on Tue Jul 29 16:32:58 2008
