3b3r

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[[Image:3b3r.jpg|left|200px]]
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{{Seed}}
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[[Image:3b3r.png|left|200px]]
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{{STRUCTURE_3b3r| PDB=3b3r | SCENE= }}
{{STRUCTURE_3b3r| PDB=3b3r | SCENE= }}
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'''Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)'''
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===Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)===
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==Overview==
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Two high-resolution structures of a double mutant of bacterial cholesterol oxidase in the presence or absence of a ligand, glycerol, are presented, showing the trajectory of glycerol as it binds in a Michaelis complex-like position in the active site. A group of three aromatic residues forces the oxidized isoalloxazine moiety to bend along the N5-N10 axis as a response to the binding of glycerol in the active site. Movement of these aromatic residues is only observed in the glycerol-bound structure, indicating that some tuning of the FAD redox potential is caused by the formation of the Michaelis complex during regular catalysis. This structural study suggests a possible mechanism of substrate-assisted flavin activation, improves our understanding of the interplay between the enzyme, its flavin cofactor and its substrate, and is of use to the future design of effective cholesterol oxidase inhibitors.
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The line below this paragraph, {{ABSTRACT_PUBMED_18029419}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18029419 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18029419}}
==About this Structure==
==About this Structure==
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[[Category: Secreted]]
[[Category: Secreted]]
[[Category: Steroid metabolism]]
[[Category: Steroid metabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:21:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:34:38 2008''

Revision as of 13:35, 29 July 2008

Image:3b3r.png

Template:STRUCTURE 3b3r

Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)

Template:ABSTRACT PUBMED 18029419

About this Structure

3B3R is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.

Reference

Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase., Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A, Protein Sci. 2007 Dec;16(12):2647-56. PMID:18029419

Page seeded by OCA on Tue Jul 29 16:34:38 2008

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