From Proteopedia
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| - | [[Image:1wao.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1wao.png|left|200px]] |
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| | {{STRUCTURE_1wao| PDB=1wao | SCENE= }} | | {{STRUCTURE_1wao| PDB=1wao | SCENE= }} |
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| - | '''PP5 STRUCTURE'''
| + | ===PP5 STRUCTURE=== |
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| - | ==Overview==
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| - | Protein phosphatase 5 (Ppp5) is a serine/threonine protein phosphatase comprising a regulatory tetratricopeptide repeat (TPR) domain N-terminal to its phosphatase domain. Ppp5 functions in signalling pathways that control cellular responses to stress, glucocorticoids and DNA damage. Its phosphatase activity is suppressed by an autoinhibited conformation maintained by the TPR domain and a C-terminal subdomain. By interacting with the TPR domain, heat shock protein 90 (Hsp90) and fatty acids including arachidonic acid stimulate phosphatase activity. Here, we describe the structure of the autoinhibited state of Ppp5, revealing mechanisms of TPR-mediated phosphatase inhibition and Hsp90- and arachidonic acid-induced stimulation of phosphatase activity. The TPR domain engages with the catalytic channel of the phosphatase domain, restricting access to the catalytic site. This autoinhibited conformation of Ppp5 is stabilised by the C-terminal alphaJ helix that contacts a region of the Hsp90-binding groove on the TPR domain. Hsp90 activates Ppp5 by disrupting TPR-phosphatase domain interactions, permitting substrate access to the constitutively active phosphatase domain, whereas arachidonic acid prompts an alternate conformation of the TPR domain, destabilising the TPR-phosphatase domain interface.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15577939}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15577939 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15577939}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Tpr]] | | [[Category: Tpr]] |
| | [[Category: X-ray structure]] | | [[Category: X-ray structure]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:23:26 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:36:51 2008'' |
Revision as of 13:36, 29 July 2008
Template:STRUCTURE 1wao
PP5 STRUCTURE
Template:ABSTRACT PUBMED 15577939
About this Structure
1WAO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis for TPR domain-mediated regulation of protein phosphatase 5., Yang J, Roe SM, Cliff MJ, Williams MA, Ladbury JE, Cohen PT, Barford D, EMBO J. 2005 Jan 12;24(1):1-10. Epub 2004 Dec 2. PMID:15577939
Page seeded by OCA on Tue Jul 29 16:36:51 2008
