1sg5

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{{STRUCTURE_1sg5| PDB=1sg5 | SCENE= }}
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'''Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination'''
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===Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination===
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==Overview==
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Rho-dependent transcription termination is an essential process for the regulation of bacterial gene expression. Thus far, only two Rho-specific inhibitors of bacterial transcription termination have been described, the psu protein from the satellite bacteriophage P4 and YaeO from Escherichia coli. Here, we report the solution structure of YaeO, the first of a Rho-specific inhibitor of transcription termination. YaeO is an acidic protein composed of an N-terminal helix and a seven-stranded beta sandwich. NMR chemical shift perturbation experiments revealed that YaeO binds proximal to the primary nucleic acid binding site of Rho. Based on the NMR titrations, a docked model of the YaeO-Rho complex was calculated. These results suggest that YaeO binds outside the Rho hexamer, acting as a competitive inhibitor of RNA binding. In vitro gel shift assays confirmed the inhibition of nucleic acid binding to Rho. Site-directed mutagenesis showed that the negative character of YaeO is essential for its function in vivo.
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{{ABSTRACT_PUBMED_17565995}}
==About this Structure==
==About this Structure==
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1SG5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG5 OCA].
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1SG5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG5 OCA].
==Reference==
==Reference==
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[[Category: Montreal-kingston bacterial structural genomics initiative]]
[[Category: Montreal-kingston bacterial structural genomics initiative]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:38:31 2008''

Revision as of 13:38, 29 July 2008

Image:1sg5.png

Template:STRUCTURE 1sg5

Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination

Template:ABSTRACT PUBMED 17565995

About this Structure

1SG5 is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structure of YaeO, a Rho-specific inhibitor of transcription termination., Gutierrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K, J Biol Chem. 2007 Aug 10;282(32):23348-53. Epub 2007 Jun 11. PMID:17565995

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