2q8y

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{{STRUCTURE_2q8y| PDB=2q8y | SCENE= }}
{{STRUCTURE_2q8y| PDB=2q8y | SCENE= }}
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'''Structural insight into the enzymatic mechanism of the phophothreonine lyase'''
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===Structural insight into the enzymatic mechanism of the phophothreonine lyase===
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==Overview==
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The OspF family of phosphothreonine lyase, including SpvC from Salmonella, irreversibly inactivates the dual-phosphorylated host MAPKs (pT-X-pY) through beta elimination. We determined crystal structures of SpvC and its complex with a phosphopeptide substrate. SpvC adopts a unique fold of alpha/beta type. The disordered N terminus harbors a canonical D motif for MAPK substrate docking. The enzyme-substrate complex structure indicates that recognition of the phosphotyrosine followed by insertion of the threonine phosphate into an arginine pocket places the phosphothreonine into the enzyme active site. This requires the conformational flexibility of pT-X-pY, which suggests that p38 (pT-G-pY) is likely the preferred physiological substrate. Structure-based biochemical and enzymatic analysis allows us to propose a general acid/base mechanism for beta elimination reaction catalyzed by the phosphothreonine lyase. The mechanism described here provides a structural understanding of MAPK inactivation by a family of pathogenic effectors conserved in plant and animal systems and may also open a new route for biological catalysis.
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The line below this paragraph, {{ABSTRACT_PUBMED_18060821}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18060821 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18060821}}
==About this Structure==
==About this Structure==
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[[Category: Alpha/beta fold]]
[[Category: Alpha/beta fold]]
[[Category: Lyase/transferase complex]]
[[Category: Lyase/transferase complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:33:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:39:05 2008''

Revision as of 13:39, 29 July 2008

Image:2q8y.png

Template:STRUCTURE 2q8y

Structural insight into the enzymatic mechanism of the phophothreonine lyase

Template:ABSTRACT PUBMED 18060821

About this Structure

2Q8Y is a Protein complex structure of sequences from Salmonella enteritidis. Full crystallographic information is available from OCA.

Reference

Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase., Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC, Shao F, Mol Cell. 2007 Dec 14;28(5):899-913. Epub 2007 Nov 29. PMID:18060821

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