This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qwj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search

OCA (Talk | contribs)
(New page: 200px<br /><applet load="1qwj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwj, resolution 2.8&Aring;" /> '''The Crystal Structure...)
Next diff →

Revision as of 22:56, 20 November 2007

Image:1qwj.gif

1qwj, resolution 2.8Å

Drag the structure with the mouse to rotate

The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase

Overview

Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase, prior to their transfer onto oligo- or polysaccharides. Here, we present, the crystal structure of the N-terminal catalytically active domain of the, murine 5-N-acetylneuraminic acid synthetase in complex with the reaction, product. In contrast to the previously solved structure of, 5-N-acetylneuraminic acid synthetase from Neisseria meningitidis and the, related CMP-KDO-synthetase of Escherichia coli, the murine enzyme is a, tetramer, which was observed with the active sites closed. In this, conformation a loop is shifted by 6A towards the active site and thus an, essential arginine residue can participate in catalysis. Furthermore, a, network of intermolecular salt-bridges and hydrogen bonds in the dimer as, well as hydrophobic interfaces between two dimers indicate a cooperative, behaviour of the enzyme. In addition, a complex regulation of the enzyme, activity is proposed that includes phosphorylation and dephosphorylation.

About this Structure

1QWJ is a Single protein structure of sequence from Mus musculus with NCC as ligand. Active as N-acylneuraminate cytidylyltransferase, with EC number 2.7.7.43 Full crystallographic information is available from OCA.

Reference

The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:14636592

Page seeded by OCA on Wed Nov 21 01:04:02 2007

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qwj"
Personal tools