1rk0

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Revision as of 23:32, 20 November 2007

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spinqualitylabelsall models 1rk0, resolution 2.61Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Mhc Class I H-2Kb Heavy Chain Complexed With beta-2 Microglobulin and Herpes Simplex Virus Glycoprotein B peptide

Overview

Major histocompatibility complex (MHC) class I variants H-2K(b) and, H-2K(bm8) differ primarily in the B pocket of the peptide-binding groove, which serves to sequester the P2 secondary anchor residue. This, polymorphism determines resistance to lethal herpes simplex virus (HSV-1), infection by modulating T cell responses to the immunodominant, glycoprotein B(498-505) epitope, HSV8. We studied the molecular basis of, these effects and confirmed that T cell receptors raised against K(b)-HSV8, cannot recognize H-2K(bm8)-HSV8. However, substitution of Ser(P2) to, Glu(P2) (peptide H2E) reversed T cell receptor (TCR) recognition;, H-2K(bm8)-H2E was recognized whereas H-2K(b)-H2E was not. Insight into the, structural basis of this discrimination was obtained by determining the, crystal structures of all four MHC class I molecules in complex with bound, peptide (pMHCs). Surprisingly, we find no concerted pMHC surface, differences that can explain the differential TCR recognition. However, a, correlation is apparent between the recognition data and the underlying, peptide-binding groove chemistry of the B pocket, revealing that secondary, anchor residues can profoundly affect TCR engagement through mechanisms, distinct from the alteration of the resting state conformation of the pMHC, surface.

About this Structure

1RK0 is a Protein complex structure of sequences from Mus musculus with NDG as ligand. Full crystallographic information is available from OCA.

Reference

Structural basis for the restoration of TCR recognition of an MHC allelic variant by peptide secondary anchor substitution., Miley MJ, Messaoudi I, Metzner BM, Wu Y, Nikolich-Zugich J, Fremont DH, J Exp Med. 2004 Dec 6;200(11):1445-54. Epub 2004 Nov 22. PMID:15557346

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