2pne
From Proteopedia
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{{STRUCTURE_2pne| PDB=2pne | SCENE= }} | {{STRUCTURE_2pne| PDB=2pne | SCENE= }} | ||
| - | + | ===Crystal Structure of the Snow Flea Antifreeze Protein=== | |
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==About this Structure== | ==About this Structure== | ||
2PNE is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNE OCA]. | 2PNE is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNE OCA]. | ||
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| + | ==Reference== | ||
| + | X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18598029 18598029] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dashnau, J.]] | [[Category: Dashnau, J.]] | ||
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[[Category: Chemical protein synthesis]] | [[Category: Chemical protein synthesis]] | ||
[[Category: Native chemical ligation]] | [[Category: Native chemical ligation]] | ||
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Revision as of 06:08, 24 September 2008
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| 2pne, resolution 0.98Å () | |||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal Structure of the Snow Flea Antifreeze Protein
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the chemically synthesized proteins d-sfAFP and l-sfAFP occurred much more readily than for l-sfAFP alone. More facile crystal formation also occurred from a quasi-racemic mixture of d-sfAFP and l-Se-sfAFP, a chemical protein analogue that contains an additional -SeCH2- moiety at one residue and thus differs slightly from the true enantiomer. Multiple wavelength anomalous dispersion (MAD) phasing from quasi-racemate crystals was then used to determine the X-ray structure of the sfAFP protein molecule. The resulting model was used to solve by molecular replacement the X-ray structure of l-sfAFP to a resolution of 0.98 A. The l-sfAFP molecule is made up of six antiparallel left-handed PPII helixes, stacked in two sets of three, to form a compact brick-like structure with one hydrophilic face and one hydrophobic face. This is a novel experimental protein structure and closely resembles a structural model proposed for sfAFP. These results illustrate the utility of total chemical synthesis combined with racemic crystallization and X-ray crystallography for determining the unknown structure of a protein.
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2PNE is a Single protein structure. Full crystallographic information is available from OCA.
Reference
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029
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